Potassium ferrocyanide trihydrate, 98.5%
Based on 1 Customer Validation
Potassium ferrocyanide trihydrate, 98.5% is a biochemical reagent. Potassium ferrocyanide trihydrate, 98.5% can be used as a food additive.
For research use only. We do not sell to patients.
- Purity: 99.7%
- CAS No.: 14459-95-1
- Formula: K4Fe(CN)6.3H2O
- Molecular Weight:422.39
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Potassium ferrocyanide trihydrate, 98.5% reacts with magnetic nanoparticles (MNPs) to generate Prussian blue in situ. Prussian blue is a substance with peroxidase-like activity that can catalyze the oxidation of luminol, thereby enhancing the chemiluminescent signal[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 14459-95-1
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Appearance Solid
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Molecular Weight 422.39
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Formula K4Fe(CN)6.3H2O
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Color Off-white to yellow
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SMILES
N#[C-][Fe+2]([C-]#N)([C-]#N)([C-]#N)([C-]#N)[C-]#N.[K+].[K+].[K+].[K+].O.O.O
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Synonyms
Potassium hexacyanoferrate(II) trihydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
H2O : 100 mg/mL (236.75 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang N, et al., In Situ Generation of Prussian Blue with Potassium Ferrocyanide to Improve the Sensitivity of Chemiluminescence Immunoassay Using Magnetic Nanoparticles as Label. Anal Chem. 2019 Apr 2;91(7):4906-4912. [Content Brief]
[2]. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS); Younes M, et al., Re-evaluation of sodium ferrocyanide (E 535), potassium ferrocyanide (E 536) and calcium ferrocyanide (E 538) as food additives. EFSA J. 2018 Jul 25;16(7):e05374. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.3675 mL | 11.8374 mL | 23.6748 mL | 59.1870 mL |
| 5 mM | 0.4735 mL | 2.3675 mL | 4.7350 mL | 11.8374 mL | |
| 10 mM | 0.2367 mL | 1.1837 mL | 2.3675 mL | 5.9187 mL | |
| 15 mM | 0.1578 mL | 0.7892 mL | 1.5783 mL | 3.9458 mL | |
| 20 mM | 0.1184 mL | 0.5919 mL | 1.1837 mL | 2.9594 mL | |
| 25 mM | 0.0947 mL | 0.4735 mL | 0.9470 mL | 2.3675 mL | |
| 30 mM | 0.0789 mL | 0.3946 mL | 0.7892 mL | 1.9729 mL | |
| 40 mM | 0.0592 mL | 0.2959 mL | 0.5919 mL | 1.4797 mL | |
| 50 mM | 0.0473 mL | 0.2367 mL | 0.4735 mL | 1.1837 mL | |
| 60 mM | 0.0395 mL | 0.1973 mL | 0.3946 mL | 0.9865 mL | |
| 80 mM | 0.0296 mL | 0.1480 mL | 0.2959 mL | 0.7398 mL | |
| 100 mM | 0.0237 mL | 0.1184 mL | 0.2367 mL | 0.5919 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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