Fluorescein-12-dGTP
Based on 1 Customer Validation
Fluorescein-12-dGTP is a fluorescein-labeled deoxyguanosine triphosphate. Fluorescein-12-dGTP incorporates into complementary allele-specific primers at SNP sites, enabling FRET with cationic conjugated polymer PFP via DNA-PFP electrostatic interactions. Fluorescein-12-dGTP incorporates into G-allele-specific primers for homozygous G/G SNP genotypes to enable genotype discrimination. Fluorescein-12-dGTP serves as a fluorescently labeled nucleotide for homogeneous solution-based SNP and FRET genotyping assays (Ex/Em = 498/517 nm).
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- Pureté : 99%
- CAS No.: 936107-90-3
- Formule: C41H41N6O20P3
- Masse moléculaire:1030.71
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Stockage:
Solution, -20°C, 2 years
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Activité biologique
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Chemical Information
- Pureté et documentation
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Help & FAQs
Activité biologique
Description
In Vitro
Fluorescein-12-dGTP (2 μM; 60 cycles of denaturation/annealing) enables clear discrimination of rs2241715 G→T SNP genotypes (homozygous G/G, heterozygous G/T, homozygous T/T) from human genomic DNA via single-base extension and PFP-mediated FRET, with genotype-specific FRET ratios ranging from 0.05 to 0.38[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 936107-90-3
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Appearance Liquid
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Masse moléculaire 1030.71
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Formule C41H41N6O20P3
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SMILES
O[C@H]1C[C@H](N(C2=C3C(NC(N)=N2)=O)C=C3C#CCNC(CCCCCNC(C4=CC=C(C(C5=C(C(OC6=C5C=CC(O)=C6)=C7)C=CC7=O)=C4)C(O)=O)=O)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Solution, -20°C, 2 years
Pureté et documentation
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Fiche technique (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fluorescein-12-dGTP Related Classifications
HY-D3310 Related Classifications
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Keywords
- Fluorescein-12-dGTP
- 936107-90-3
- Fluorescent Dye
- human genomic DNA
- deoxyguanosine triphosphate
- SNP genotyping assays
- SNP sites
- FRET donor/acceptor partner
- homozygous G/G SNP genotypes
- cationic conjugated polymer PFP
- DNA-PFP electrostatic interactions
- G-allele-specific primers
- single-base extension reactions
- Inhibitor
- inhibitor
- inhibit