2. Immunology/Inflammation
  3. Cyclic GMP-AMP Synthase
  4. G140

G140 is a potent and selective inhibitor of cyclic GMP-AMP synthase (cGAS), with IC50s of 14.0 nM and 442 nM for h-cGAS and m-cGAS, respectively. G140 has anti-inflammatory activity.

For research use only. We do not sell to patients.

G140 Chemical Structure

G140 Chemical Structure

CAS No. : 2369751-07-3

Size Price Stock Quantity
1 mg USD 90 In-stock
5 mg USD 270 In-stock
10 mg USD 420 In-stock
25 mg USD 840 In-stock
50 mg USD 1350 In-stock
100 mg USD 2150 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

G140 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE G140

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

G140 is a potent and selective inhibitor of cyclic GMP-AMP synthase (cGAS), with IC50s of 14.0 nM and 442 nM for h-cGAS and m-cGAS, respectively. G140 has anti-inflammatory activity[1].

IC50 & Target

IC50: 14.0 nM (h-cGAS), 442 nM (m-cGAS)[1]
In Vitro

G140 (0.1-100 μM; 4 h) inhibits h-cGAS in a dose-dependent manner with IC50s of 1.70 and 0.86?μM in human THP1 and primary macrophages, respectively[1].
? G140 (0.1-100 μM; 24 h) inhibits cells viability with a LD50 of >100 μM in THP1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

G140 Related Antibodies
Molecular Weight

379.24

Formula

C17H16Cl2N4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(N(C1)CCC(N2)=C1C3=C2C(Cl)=C(Cl)C=C3C4=NN(C)C=C4)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (659.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6369 mL 13.1843 mL 26.3685 mL
5 mM 0.5274 mL 2.6369 mL 5.2737 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (5.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6369 mL 13.1843 mL 26.3685 mL 65.9213 mL
5 mM 0.5274 mL 2.6369 mL 5.2737 mL 13.1843 mL
10 mM 0.2637 mL 1.3184 mL 2.6369 mL 6.5921 mL
15 mM 0.1758 mL 0.8790 mL 1.7579 mL 4.3948 mL
20 mM 0.1318 mL 0.6592 mL 1.3184 mL 3.2961 mL
25 mM 0.1055 mL 0.5274 mL 1.0547 mL 2.6369 mL
30 mM 0.0879 mL 0.4395 mL 0.8790 mL 2.1974 mL
40 mM 0.0659 mL 0.3296 mL 0.6592 mL 1.6480 mL
50 mM 0.0527 mL 0.2637 mL 0.5274 mL 1.3184 mL
60 mM 0.0439 mL 0.2197 mL 0.4395 mL 1.0987 mL
80 mM 0.0330 mL 0.1648 mL 0.3296 mL 0.8240 mL
100 mM 0.0264 mL 0.1318 mL 0.2637 mL 0.6592 mL
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G140 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G1402369751-07-3G 140G-140Cyclic GMP-AMP SynthasecGAScyclicGMP-AMPsynthaseanti-inflammatoryInhibitorinhibitorinhibit

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