Canine Factor IX
Based on 1 Customer Validation
Canine Factor IX is a functional coagulation factor. Canine Factor IX shortens whole blood clotting time and activated partial thromboplastin time, and restores coagulation function. After intramuscular administration via rAAV vector in hemophilia B dogs, Canine Factor IX maintains a sustained circulating level for at least 1 year, partially corrects the hemophilia B phenotype in a dose-dependent manner, and its plasma expression persists for more than 17 months. Canine Factor IX is applicable to relevant research on hemophilia B.
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- No. CAS: 9001-28-9
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Canine Factor IX (104-105 vector particles/cell; 36 h post-transduction) was secreted in biologically active form following transduction of HEK-293 cells with AAV-CMV-cF.IX vector, measurable via ELISA of cell supernatants[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK-293 cells
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Concentration:104, 105 vector particles/cell
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Incubation Time:36 h post-transduction
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Result:Resulted in detectable secretion of biologically active canine Factor IX into cell supernatants.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Outbred dogs (male and female; 2, 4, 8 months old; 5.7-20 kg; spontaneous severe hemophilia B with undetectable endogenous F.IX)[2]
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Dosage:1.3 × 1011; 1.1 × 1012; 3.4 × 1012; 3 × 1012; 8.5 × 1012 vector particles/kg
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Administration:i.m.; single dose
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Result:Was stably expressed in plasma in a dose-dependent manner, reaching up to 69 ng/ml, WBCT and APTT were persistently shortened, immune responses against cFIX were absent or transient, and no local or systemic toxicity was observed.
Chemical Information
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No. CAS 9001-28-9
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Appearance Solid
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Color White to off-white
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SMILES
[Canine Factor IX]
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Envío
Shipping with dry ice.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Chao H, et al. Persistent expression of canine factor IX in hemophilia B canines. Gene Ther. 1999;6(10):1695-1704. [Content Brief]
[2]. Herzog RW, et al. Long-term correction of canine hemophilia B by gene transfer of blood coagulation factor IX mediated by adeno-associated viral vector. Nat Med. 1999;5(1):56-63. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Canine Factor IX Related Classifications
HY-E70541B Related Classifications
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