PEG2000-DMPE
Based on 1 Customer Validation
PEG2000-DMPE is a polyethylene glycol-lipid conjugate combined with phosphatidylethanolamine. PEG2000-DMPE undergoes a conformational transition from mushroom-like to brush-like at a concentration of 7 mol%, thereby exerting multiple functions such as inhibitor, stabilizer and surface defect modifier. PEG2000-DMPE can form a steric hindrance barrier on the vesicle surface to block calcium ion-induced liposome fusion, and dynamically regulate the fusion process through spontaneous transfer rate. PEG2000-DMPE can delay the thinning of foam thin liquid films and smoothly seal surface defects of solid support layers. PEG2000-DMPE can be used in the design and related research of stealth liposomes and micelles.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.87%
- Fòrmula: C37H72NO11P
- Peso molecular:2676.25
-
Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
In Vitro
PEG2000-DMPE (0.5-2 mole %; 5 min) inhibits calcium-induced fusion of DOPE:POPS LUVs in a dose-dependent manner, with 2 mole % incorporation completely blocking fusion[1].
PEG2000-DMPE (2 mole %; 60 min) has its fusion-blocking effect reversed by addition of POPC sink liposomes, which restores the fusogenic activity of DOPE:POPS LUVs, allowing calcium-induced fusion to proceed after a short lag phase[1].
PEG2000-DMPE (2-10 mole %; 60 min) increases the lag phase before recovery of fusogenic activity in DOPE:POPS LUVs with higher initial incorporation, with 10 mole % preventing fusion recovery entirely even with a POPC sink[1].
PEG2000-DMPE (≤10 mol%, 20 mol%, 35 mol%, 80 mol%) induces conversion of DMPC liposomes to micelles, with complete transition occurring at 35 mol% PEG2000-DMPE in aqueous dispersions at 37 °C, pH 6.8-7.0, and 0.5 M NaCl[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
Appearance Solid
-
Peso molecular 2676.25
-
Fòrmula C37H72NO11P
-
Color White to off-white
-
SMILES
CCCCCCCCCCCCCC(O[C@](COP(OCCNC(OCCOC)=O)(O)=O)([H])COC(CCCCCCCCCCCCC)=O)=O
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
Ethanol : 100 mg/mL (37.37 mM; Need ultrasonic)
DMSO : 100 mg/mL (37.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (0.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (0.93 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (278 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. Holland JW, Hui C, Cullis PR, et al.. Poly(ethylene glycol)--lipid conjugates regulate the calcium-induced fusion of liposomes composed of phosphatidylethanolamine and phosphatidylserine. Biochemistry. 1996 Feb 27;35(8):2618-24. [Content Brief]
[2]. Georgiev GA, Sarker DK, Al-Hanbali O, et al.. Effects of poly (ethylene glycol) chains conformational transition on the properties of mixed DMPC/DMPE-PEG thin liquid films and monolayers. Colloids and surfaces. B, Biointerfaces. 2007 Oct 01;59(2):184-93. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 0.3737 mL | 1.8683 mL | 3.7366 mL | 9.3414 mL |
| 5 mM | 0.0747 mL | 0.3737 mL | 0.7473 mL | 1.8683 mL | |
| 10 mM | 0.0374 mL | 0.1868 mL | 0.3737 mL | 0.9341 mL | |
| 15 mM | 0.0249 mL | 0.1246 mL | 0.2491 mL | 0.6228 mL | |
| 20 mM | 0.0187 mL | 0.0934 mL | 0.1868 mL | 0.4671 mL | |
| 25 mM | 0.0149 mL | 0.0747 mL | 0.1495 mL | 0.3737 mL | |
| 30 mM | 0.0125 mL | 0.0623 mL | 0.1246 mL | 0.3114 mL |
PEG2000-DMPE Related Classifications
HY-112768 Related Classifications
Powered by Bioz