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  3. Gol-NTR

Gol-NTR is a Golgi-targetable probe with high selectivity and sensitivity. Gol-NTR is Nitroreductase (NTR)-activated and has visualization acute lung injury (ALI) and repair function. Gol-NTR has a low detection limit of 54.8 ng/mL. Gol-NTR can be used for the research for monitoring and assessing research response of sepsis-induced ALI.

For research use only. We do not sell to patients.

Gol-NTR Chemical Structure

Gol-NTR Chemical Structure

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Description

Gol-NTR is a Golgi-targetable probe with high selectivity and sensitivity. Gol-NTR is Nitroreductase (NTR)-activated and has visualization acute lung injury (ALI) and repair function. Gol-NTR has a low detection limit of 54.8 ng/mL. Gol-NTR can be used for the research for monitoring and assessing research response of sepsis-induced ALI[1].

In Vitro

Fluorescent labeling of NTR by Gol-NTR[1]
(1) Prepare 1.0 mM Gol-NTR stock solution with DMSO solution.
(2) Dilute the stock solution with DMSO solution to prepare 5.0 μM Gol-NTR working solution.
(3) Mix 5.0 μM Gol-NTR with 50 μM NADH in PBS buffer (10 mM, pH 7.4) containing 5% DMSO, and then add appropriate NTR.
(4) After incubation at 37℃ for 30 min, the spectra was recorded at 405 nm (slit width: dex/dem=5/5 nm).
Fluorescence labeling of NTR in A549 cells by Gol-NTR[1]
(1) A549 cells were cultured at different oxygen concentrations (1%, 5%, 10%, 15% and 20% O2) for 8 h.
(2) A549 cells were washed with phosphate buffered saline (PBS).
(3) A549 cells were treated with 5.0 μM Gol-NTR for 1 h.
(4) Fluorescence images of A549 cells were observed using confocal fluorescence imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In Vivo Imaging[1]
(1) C57BL/6 male mice (6-8 weeks old, weight 20-22 g) were pre injected with 300 μL DMOG (25 mg/mL), after 24 h, intraperitoneal injection of 300 μL LPS (10 mg/kg) for 6 h.
(2) Mice were killed by cervical vertebra dislocation and lung organs were collected.
(3) After washing with PBS, incubate with 50 μM Gol-NTR in PBS for 1 h.
(4) After washing with PBS, fluorescence imaging was performed on a small animal imaging system (excitation wavelength of 420 nm and emission wavelength of 510 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

467.40

Formula

C24H16F3N3O4

Emission (Em)

730

Excitation (Ex)

680

SMILES

FC(F)(C1=NC(C=C(NC(OCC2=CC=C([N+]([O-])=O)C=C2)=O)C=C3)=C3C(C4=CC=CC=C4)=C1)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Gol-NTR Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Gol-NTR
Cat. No.:
HY-151537
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