Gol-NTR

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fluorescent labeling of NTR by Gol-NTR^[1]
(1) Prepare 1.0 mM Gol-NTR stock solution with DMSO solution.
(2) Dilute the stock solution with DMSO solution to prepare 5.0 μM Gol-NTR working solution.
(3) Mix 5.0 μM Gol-NTR with 50 μM NADH in PBS buffer (10 mM, pH 7.4) containing 5% DMSO, and then add appropriate NTR.
(4) After incubation at 37°C for 30 min, the spectra was recorded at 405 nm (slit width: d_ex/d_em=5/5 nm).
Fluorescence labeling of NTR in A549 cells by Gol-NTR^[1]
(1) A549 cells were cultured at different oxygen concentrations (1%, 5%, 10%, 15% and 20% O₂) for 8 h.
(2) A549 cells were washed with phosphate buffered saline (PBS).
(3) A549 cells were treated with 5.0 μM Gol-NTR for 1 h.
(4) Fluorescence images of A549 cells were observed using confocal fluorescence imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.