Hirudin (54-65)
Based on 1 Customer Validation
Hirudin (54-65) is a thrombin antagonist and YAP suppressor with anticoagulatory properties.Hirudin (54-65) blocks thrombin's anion binding site, acts on soluble and thrombus-bound thrombin.Hirudin (54-65) suppresses thrombin-induced profibrotic YAP activity, reduces YAP expression, nuclear translocation, and downstream effector signaling in vascular endothelial cells.Hirudin (54-65) ameliorates obstructive cholestasis, attenuates liver fibrosis symptoms, fibrosis-associated angiogenesis, and endothelial-to-mesenchymal transition.Hirudin (54-65) reduces liver inflammation and tissue hypoxia.Hirudin (54-65) promotes extracellular calcium influx through L-type calcium channels in canine coronary artery smooth muscle, mediates contraction.Hirudin (54-65) induces endothelium-independent contraction of canine coronary arterial segments; this response is not affected by indomethacin pretreatment.Hirudin (54-65) can be used for the research of liver obstructive cholestasis, liver fibrosis.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 99.20%
- CAS No.: 113274-56-9
- 화학식: C66H93N13O25
- 분자량:1468.52
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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HY-10787
XimelagatranThrombin Inhibitor -
HY-B0375A
Argatroban monohydrateThrombin Inhibitor -
HY-P4803
PAR-2 (1-6) (human)PAR2 Agonist -
HY-10277
Odiparcilβ-D-xyloside Derivative -
HY-10274A
Dabigatran etexilate mesylateAntithrombotic Agent -
HY-P1000
TRAP-14Thrombin Receptor Agonist -
HY-10787R
Ximelagatran (Standard)Reference Standard -
HY-10274AR
Dabigatran etexilate mesylate (Standard)Antithrombotic Agent -
HY-B0375AR
Argatroban monohydrate (Standard)Thrombin Inhibitor -
HY-10277R
Odiparcil (Standard)Reference Standard -
HY-P1536
Thrombin Receptor Activator for Peptide 5 (TRAP-5) -
HY-P4451
TRAP-5 amidePAR 1 Agonist
Biological Activity
제품 설명
IC50 & Target
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L-type calcium channel |
In Vitro
Hirudin (54-65) (10-10-10-6 mol/L) induces concentration-dependent, non-endothelium-dependent contraction in isolated canine left circumflex coronary artery segments in vitro. At the concentration of 10-6 mol/L, the maximum tension elevation reaches 33.6% in the endothelium-intact group and 31.8% in the endothelium-denuded group[2].
Hirudin (54-65) (10-8-10-6 mol/L) exerts a concentration-dependent contractile effect on canine left circumflex coronary artery segments with intact endothelium, and this effect is almost completely inhibited by pretreatment with 10-4 mol/L Verapamil (HY-14275) or Nifedipine (HY-B0284) for 60 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
- Fibrosis/Collagen Morphometry
- RNA extraction experimental
- Cytoplasmic-Nuclear Fractionated Protein Extraction
- Collagen: Sirius Red Staining
- Human pluripotent stem cell endothelial-cell differentiation
- Vascular/Branching Fractal Analysis
- Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
- Endothelial Tube Formation Assay
- Research Protocol for Inflammation-related Diseases
- Ca2+ Staining Technique
- Endothelial Cell Migration/Angiogenic Sprouting Assay
- Liver Histomorphometry
Chemical Information
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CAS No. 113274-56-9
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Appearance Solid
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분자량 1468.52
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화학식 C66H93N13O25
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Color White to off-white
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Sequence
Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln
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Sequence Shortening
GDFEEIPEEYLQ
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (68.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
순도&문서
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Data Sheet (286 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang X, et al. Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis. Exp Biol Med (Maywood). 2023;248(14):1242-1253. [Content Brief]
[2]. Sorajja P, et al. Hirudin (desulfated, 54-65) contracts canine coronary arteries: extracellular calcium influx mediates hirudin-induced contractions. J Surg Res. 2004;121(1):38-41. [Content Brief]
[3]. Acquasaliente L, et al. From haemadin to haemanorm: Synthesis and characterization of full-length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm). Protein Sci. 2023;32(12):e4825. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.6810 mL | 3.4048 mL | 6.8096 mL | 17.0239 mL |
| 5 mM | 0.1362 mL | 0.6810 mL | 1.3619 mL | 3.4048 mL | |
| 10 mM | 0.0681 mL | 0.3405 mL | 0.6810 mL | 1.7024 mL | |
| 15 mM | 0.0454 mL | 0.2270 mL | 0.4540 mL | 1.1349 mL | |
| 20 mM | 0.0340 mL | 0.1702 mL | 0.3405 mL | 0.8512 mL | |
| 25 mM | 0.0272 mL | 0.1362 mL | 0.2724 mL | 0.6810 mL | |
| 30 mM | 0.0227 mL | 0.1135 mL | 0.2270 mL | 0.5675 mL | |
| 40 mM | 0.0170 mL | 0.0851 mL | 0.1702 mL | 0.4256 mL | |
| 50 mM | 0.0136 mL | 0.0681 mL | 0.1362 mL | 0.3405 mL | |
| 60 mM | 0.0113 mL | 0.0567 mL | 0.1135 mL | 0.2837 mL |
Hirudin (54-65) Related Classifications
HY-P1636 Related Classifications
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Keywords
- Hirudin (54-65)
- 113274-56-9
- Thrombin
- YAP
- Calcium Channel
- Yes-associated protein
- Ca2+ channels
- Ca channels
- canine left circumflex coronary artery segments
- L-type calcium channels
- canine coronary artery smooth muscle
- obstructive cholestasis
- endothelial-to-mesenchymal transition
- thrombin
- vascular endothelial cells
- liver fibrosis
- verapamil
- Inhibitor
- inhibitor
- inhibit