Hirudin (54-65) 

Hirudin (54-65) is a thrombin antagonist and YAP suppressor with anticoagulatory properties.Hirudin (54-65) blocks thrombin's anion binding site, acts on soluble and thrombus-bound thrombin.Hirudin (54-65) suppresses thrombin-induced profibrotic YAP activity, reduces YAP expression, nuclear translocation, and downstream effector signaling in vascular endothelial cells.Hirudin (54-65) ameliorates obstructive cholestasis, attenuates liver fibrosis symptoms, fibrosis-associated angiogenesis, and endothelial-to-mesenchymal transition.Hirudin (54-65) reduces liver inflammation and tissue hypoxia.Hirudin (54-65) promotes extracellular calcium influx through L-type calcium channels in canine coronary artery smooth muscle, mediates contraction.Hirudin (54-65) induces endothelium-independent contraction of canine coronary arterial segments; this response is not affected by indomethacin pretreatment.Hirudin (54-65) can be used for the research of liver obstructive cholestasis, liver fibrosis^[1].

Hirudin (54-65) (10^-10-10^-6 mol/L) induces concentration-dependent, non-endothelium-dependent contraction in isolated canine left circumflex coronary artery segments in vitro. At the concentration of 10^-6 mol/L, the maximum tension elevation reaches 33.6% in the endothelium-intact group and 31.8% in the endothelium-denuded group^[2].
Hirudin (54-65) (10^-8-10^-6 mol/L) exerts a concentration-dependent contractile effect on canine left circumflex coronary artery segments with intact endothelium, and this effect is almost completely inhibited by pretreatment with 10^-4 mol/L Verapamil (HY-14275) or Nifedipine (HY-B0284) for 60 min^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1468.52

C₆₆H₉₃N₁₃O₂₅

113274-56-9

Solid

White to off-white

Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln

GDFEEIPEEYLQ

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Yang X, et al. Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis. Exp Biol Med (Maywood). 2023;248(14):1242-1253.  [Content Brief]

  [2]. Sorajja P, et al. Hirudin (desulfated, 54-65) contracts canine coronary arteries: extracellular calcium influx mediates hirudin-induced contractions. J Surg Res. 2004;121(1):38-41.  [Content Brief]

  [3]. Acquasaliente L, et al. From haemadin to haemanorm: Synthesis and characterization of full-length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm). Protein Sci. 2023;32(12):e4825.  [Content Brief]