Hirudin (54-65)
Based on 1 Customer Validation
Hirudin (54-65) is a thrombin antagonist and YAP suppressor with anticoagulatory properties.Hirudin (54-65) blocks thrombin's anion binding site, acts on soluble and thrombus-bound thrombin.Hirudin (54-65) suppresses thrombin-induced profibrotic YAP activity, reduces YAP expression, nuclear translocation, and downstream effector signaling in vascular endothelial cells.Hirudin (54-65) ameliorates obstructive cholestasis, attenuates liver fibrosis symptoms, fibrosis-associated angiogenesis, and endothelial-to-mesenchymal transition.Hirudin (54-65) reduces liver inflammation and tissue hypoxia.Hirudin (54-65) promotes extracellular calcium influx through L-type calcium channels in canine coronary artery smooth muscle, mediates contraction.Hirudin (54-65) induces endothelium-independent contraction of canine coronary arterial segments; this response is not affected by indomethacin pretreatment.Hirudin (54-65) can be used for the research of liver obstructive cholestasis, liver fibrosis.
For research use only. We do not sell to patients.
- Purity: 99.20%
- CAS No.: 113274-56-9
- Formula: C66H93N13O25
- Molecular Weight:1468.52
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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L-type calcium channel |
Hirudin (54-65) (10-10-10-6 mol/L) induces concentration-dependent, non-endothelium-dependent contraction in isolated canine left circumflex coronary artery segments in vitro. At the concentration of 10-6 mol/L, the maximum tension elevation reaches 33.6% in the endothelium-intact group and 31.8% in the endothelium-denuded group[2].
Hirudin (54-65) (10-8-10-6 mol/L) exerts a concentration-dependent contractile effect on canine left circumflex coronary artery segments with intact endothelium, and this effect is almost completely inhibited by pretreatment with 10-4 mol/L Verapamil (HY-14275) or Nifedipine (HY-B0284) for 60 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 113274-56-9
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Appearance Solid
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Molecular Weight 1468.52
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Formula C66H93N13O25
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Color White to off-white
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Sequence
Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-Leu-Gln
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Sequence Shortening
GDFEEIPEEYLQ
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (68.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang X, et al. Targeting endothelial coagulation signaling ameliorates liver obstructive cholestasis and dysfunctional angiogenesis. Exp Biol Med (Maywood). 2023;248(14):1242-1253. [Content Brief]
[2]. Sorajja P, et al. Hirudin (desulfated, 54-65) contracts canine coronary arteries: extracellular calcium influx mediates hirudin-induced contractions. J Surg Res. 2004;121(1):38-41. [Content Brief]
[3]. Acquasaliente L, et al. From haemadin to haemanorm: Synthesis and characterization of full-length haemadin from the leech Haemadipsa sylvestris and of a novel bivalent, highly potent thrombin inhibitor (haemanorm). Protein Sci. 2023;32(12):e4825. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.6810 mL | 3.4048 mL | 6.8096 mL | 17.0239 mL |
| 5 mM | 0.1362 mL | 0.6810 mL | 1.3619 mL | 3.4048 mL | |
| 10 mM | 0.0681 mL | 0.3405 mL | 0.6810 mL | 1.7024 mL | |
| 15 mM | 0.0454 mL | 0.2270 mL | 0.4540 mL | 1.1349 mL | |
| 20 mM | 0.0340 mL | 0.1702 mL | 0.3405 mL | 0.8512 mL | |
| 25 mM | 0.0272 mL | 0.1362 mL | 0.2724 mL | 0.6810 mL | |
| 30 mM | 0.0227 mL | 0.1135 mL | 0.2270 mL | 0.5675 mL | |
| 40 mM | 0.0170 mL | 0.0851 mL | 0.1702 mL | 0.4256 mL | |
| 50 mM | 0.0136 mL | 0.0681 mL | 0.1362 mL | 0.3405 mL | |
| 60 mM | 0.0113 mL | 0.0567 mL | 0.1135 mL | 0.2837 mL |
Hirudin (54-65) Related Classifications
HY-P1636 Related Classifications
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- Hirudin (54-65)
- 113274-56-9
- Thrombin
- YAP
- Calcium Channel
- canine left circumflex coronary artery segments
- L-type calcium channels
- canine coronary artery smooth muscle
- obstructive cholestasis
- endothelial-to-mesenchymal transition
- thrombin
- vascular endothelial cells
- liver fibrosis
- verapamil
- Inhibitor
- inhibitor
- inhibit