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  4. huDMB5F3

huDMB5F3  (Synonyms: DMB5F3; chDMB5F3)

Cat. No.: HY-P992374
Technical Support

huDMB5F3 (DMB5F3; chDMB5F3) is a human monoclonal antibody against human CD227/MUC1, with a Ka value of 5.89 pM for its human target. huDMB5F3 enters MUC1-positive cancer cells via a temperature-dependent internalization process. huDMB5F3 induces cytotoxicity in MUC1-positive cancer cells. huDMB5F3 can be used in the research of various cancers including breast cancer, pancreatic cancer and gastric cancer.

For research use only. We do not sell to patients.

huDMB5F3

huDMB5F3 Chemical Structure

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Description

huDMB5F3 (DMB5F3; chDMB5F3) is a human monoclonal antibody against human CD227/MUC1, with a Ka value of 5.89 pM for its human target. huDMB5F3 enters MUC1-positive cancer cells via a temperature-dependent internalization process. huDMB5F3 induces cytotoxicity in MUC1-positive cancer cells. huDMB5F3 can be used in the research of various cancers including breast cancer, pancreatic cancer and gastric cancer[1].

Species Reactivity

Human
IC50 & Target[1]

MUC1

5.89 pM (Kd)

In Vitro

huDMB5F3 (0.4-7 nM; 4-min association, 30-min dissociation) binds to recombinant MUC1-X protein with extremely high affinity (Kd = 5.89 pM), whereas the monovalent Fab fragment exhibits 100-fold lower affinity due to reduced avidity (Kd = 4.84 nM)[1].
huDMB5F3 (62 ng/mL-4 μg/mL; 1-h primary antibody incubation, 45-min secondary antibody incubation) binds specifically to human breast cancer ZR75 cells expressing MUC1, and a binding signal is detectable at concentrations as low as 62 ng/mL (0.4 nM)[1].
huDMB5F3 (incubated at 4°C or 37°C) is efficiently internalized into MUC1-expressing cells in a temperature-dependent manner, localizing to the cell membrane at 4°C and entering the intracellular space at 37°C[1].
huDMB5F3 (0.001-1000 pM; incubated with cells following 5-h seeding) potently and selectively kills MUC1-expressing DA3-TM, T47D, Colo357 and ZR75 cancer cells, with an IC50 value of approximately 16 pM in Colo357 cells and approximately 3 pM in ZR75 cells; soluble MUC1-Xex abrogates its cytotoxicity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

huDMB5F3 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: DA3-TM (mouse mammary tumor cells expressing human MUC1-TM), T47D human breast cancer cells, Colo357 human pancreatic cancer cells, ZR75 human breast cancer cells
Concentration: 0.001, 0.01, 0.1, 1, 10, 100, 1000 pM
Incubation Time: incubated with cells following 5-h seeding
Result: Showed potent cytotoxicity against MUC1-positive cells: DA3-TM cells were highly sensitive with cytocidal activity at picomolar concentrations.
Caused extensive cell killing of T47D cells at picomolar concentrations.
Resulted in an IC50 of approximately 16 pM for Colo357 cells.
Resulted in an IC50 of approximately 3 pM for ZR75 cells.
Had cytotoxicity abolished by soluble recombinant MUC1-Xex protein, confirming specificity.
Showed no effect on MUC1-negative DA3-PAR cells.
Gene ID

4582  [NCBI]

Accession
Target

MUC1
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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huDMB5F3 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

huDMB5F3DMB5F3 chDMB5F3Mucinα subunitsgastric cancerMUC1-positive cancer cellsMUC1 SEA domainβ subunitsprostate cancerbreast cancerovarian cancerpancreatic cancerMUC1-positive breast cancer cellsInhibitorinhibitorinhibit

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