Biotin-itaconate
Based on 1 Customer Validation
Biotin-itaconate is a biotin-labeled itaconic anhydride that targets PD-L1. Biotin-itaconate acts as a biotin probe for in vitro alkylation assays to identify itaconate-modified protein substrates. Biotin-itaconate is applicable to cancer research.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 93.23%
- CAS 番号: 3086566-93-7
- 分子式: C25H42N4O9S
- 分子量:574.69
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保管条件:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Biotin-itaconate (50 μM; 4 h) covalently alkylates the Cys272 residue of wild-type PD-L1 in HEK293T cells, while the C272A mutation abolishes this alkylation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T cells transfected with hemagglutinin (HA)-tagged wild-type (WT) programmed cell death ligand 1 (PD-L1) or HA-tagged PD-L1 C272A mutant plasmids
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Concentration:50 μM
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Incubation Time:4 h
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Result:Labeled WT HA-PD-L1, as detected by streptavidin blotting of immunoprecipitated protein.
Significantly decreased the level of labeling compared to WT PD-L1 when PD-L1 carried the Cys272 residue mutation.
化学情報
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CAS 番号 3086566-93-7
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性状 Oil
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分子量 574.69
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分子式 C25H42N4O9S
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Color Off-white to light brown
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SMILES
OC(C(CC(NCCOCCOCCOCCOCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)=C)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (174.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (267 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7401 mL | 8.7003 mL | 17.4007 mL | 43.5017 mL |
| 5 mM | 0.3480 mL | 1.7401 mL | 3.4801 mL | 8.7003 mL | |
| 10 mM | 0.1740 mL | 0.8700 mL | 1.7401 mL | 4.3502 mL | |
| 15 mM | 0.1160 mL | 0.5800 mL | 1.1600 mL | 2.9001 mL | |
| 20 mM | 0.0870 mL | 0.4350 mL | 0.8700 mL | 2.1751 mL | |
| 25 mM | 0.0696 mL | 0.3480 mL | 0.6960 mL | 1.7401 mL | |
| 30 mM | 0.0580 mL | 0.2900 mL | 0.5800 mL | 1.4501 mL | |
| 40 mM | 0.0435 mL | 0.2175 mL | 0.4350 mL | 1.0875 mL | |
| 50 mM | 0.0348 mL | 0.1740 mL | 0.3480 mL | 0.8700 mL | |
| 60 mM | 0.0290 mL | 0.1450 mL | 0.2900 mL | 0.7250 mL | |
| 80 mM | 0.0218 mL | 0.1088 mL | 0.2175 mL | 0.5438 mL | |
| 100 mM | 0.0174 mL | 0.0870 mL | 0.1740 mL | 0.4350 mL |
Biotin-itaconate 関連分類
HY-185447 関連分類
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