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製品番号: HY-D3168

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PCL-2

PCL-2 is a reactive oxygen species (ROS)-responsive fluorescent probe. PCL-2 reacts with ROS to release 6-hydroxy-2-cyanobenzothiazole (HCBT), which then reacts with D-cysteine to form firefly luciferin in situ. PCL-2 must be used in conjunction with IETDC (HY-D3169) to detect the co-existence of hydrogen peroxide and caspase 8. PCL-2 is applicable to studies related to acute inflammation.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 1421277-81-7
分子式: C₁₅H₁₁BN₂O₃S
分子量:310.14

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

PCL-2 構造式

CAS 番号: 1421277-81-7

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

PCL-2 (5 μM; 5, 20, 40, or 60 min) selectively produces a ca. 50-fold increase in bioluminescent signal in response to H₂O₂, but not other biologically relevant ROS, in a cell-free system^[1].

PCL-2 (5 μM; 60 min) produces a linearly increasing bioluminescent signal in response to 1-100 μM H₂O₂ in a cell-free system^[1].

PCL-2 (25 μM; 2 h) produces a linearly increasing, statistically significant bioluminescent signal in response to 0-100 μM H₂O₂ in PC3M-luc cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Mice

PCL-2 (0.05 μmol; i.p.) produces a dose-dependent, 10-fold maximum bioluminescent turn-on signal in response to exogenous hydrogen peroxide in healthy FVB-luc⁺ mice, which is attenuated by a hydrogen peroxide scavenger^[1].

Mice

PCL-2 (0.05 μmol; i.p.) detects a ~3.7-fold increase in endogenous hydrogen peroxide (H₂O₂) levels during lipopolysaccharide-induced acute inflammation in female FVB-luc+ mice^[1].

Mice

PCL-2 (0.05 μmol; i.p.), in combination with IETDC, detects a ~2.7-fold increase in simultaneous endogenous hydrogen peroxide and caspase 8 activity during LPS (HY-D1056)-induced acute inflammation in female FVB-luc+ mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol D-cysteine)
Administration:

intraperitoneal injection
Result:

Produced a ~3.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~55% when co-administered with apocynin relative to signal from lipopolysaccharide alone.

Animal Model:

FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol IETDC)
Administration:

intraperitoneal injection
Result:

Produced a ~2.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~30% when co-administered with ascorbic acid and z-VD(OMe)-OPh relative to signal from lipopolysaccharide alone.

Related Protocols :

化学情報

CAS 番号 1421277-81-7
分子量 310.14
分子式 C₁₅H₁₁BN₂O₃S
SMILES

N#CC1=NC2=CC=C(C=C2S1)OCC3=CC=C(C=C3)B(O)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

生物活性
- 製品説明
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献

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PCL-2

生物活性

製品説明

体外実験

体内実験

化学情報

純度とドキュメンテーション

参考文献

Calculators

Help & FAQs

PCL-2 関連分類

HY-D3168 関連分類

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