QM-B-CF

QM-B-CF (2 h) generates detectable chemiluminescent signals in cells after incubation with SKOV-3 or 4T1 cells for 2 h followed by irradiation at 500 mW/cm² for 1 min^[1].
QM-B-CF (2 h) generates a detectable fluorescent signal that can be regulated by the cellular redox state. After incubation with 4T1 cells for 2 h, the highest fluorescence intensity is observed in BSO-treated cells^[1].

Guide (The following is our recommended experimental protocol. This protocol serves only as a reference guide, and specific operations should be adjusted according to your actual requirements).
1. Stock Solution Preparation
QM-B-CF is dissolved in anhydrous DMSO to prepare a 1-5 mM stock solution, which is stored at -20℃ away from light.
The working solution is solubilized/solubilized-enhanced using PBS (pH 7.4) containing 20 eq (2,3,6-tri-O-methyl)-β-cyclodextrin (T-β-CD), with sonication to promote dissolution (final DMSO content ≤ 0.5-1%).
2. In Vitro Fluorescence & CL Assay (96-Well Black Plate)
2.1 Prepare a 50 μM working solution (containing 20 eq T-β-CD) using PBS (100 mM, pH 7.4).
2.2 Add H₂O₂ (300 μM); incubate at 37℃.
2.3 Fluorescence measurement: Ex = 514 nm, Em = 600 nm (or scan within 550-700 nm).
2.4 CL measurement: Incubate with H₂O₂ for 30 min → irradiate with white light LED (500 mW/cm², 1 min) → immediately collect CL signals using ImageQuant LAS4000 or IVIS (acq 1 min).
2.5 Controls: No H₂O₂ added or no light irradiation.
3. Cellular Fluorescence Imaging (4T1 / Skov-3)
3.1 Seed cells in glass-bottom dishes (approximately 2.5×10⁴/well) and culture for 12-24 h.
3.2 Replace with serum-free/low-serum medium, then add QM-B-CF to a final concentration of 10-20 μM (containing 20 eq T-β-CD).
3.3 Incubate at 37℃ with 5% CO₂ for 2 h.
3.4 Wash 3 times with PBS (pH 7.4).
3.5 Confocal imaging: Use a 514 nm laser for Ex, and collect Em signals within 600-650 nm (red channel).
3.6 Inhibition group: Pre-incubate with NAC (HY-B0215) (5 mM, 30-60 min) before adding the probe.
4. Cellular CL Assay (96-Well Black-Walled Plate)
4.1 Incubate cells with QM-B-CF (containing T-β-CD) for 2-3 h (4T1 cells with high endogenous H₂O₂ or pre-treated with exogenous H₂O₂).
4.2 Wash 3 times with PBS (pH 7.4).
4.3 Immediately after white light irradiation (500 mW/cm², 1 min), collect CL signals using LAS4000 (5 min acq).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.