SNAP-Biotin
SNAP-Biotin is a cell-permeable SNAP-tag-targeted labeling reagent. SNAP-Biotin undergoes an irreversible covalent reaction with SNAP-tag to transfer biotin to the tag. SNAP-Biotin enables the detection of SNAP-tag fusion proteins via a streptavidin-HRP immunoblotting system and achieves biotinylation of SNAP-tag fusion proteins. SNAP-Biotin is applicable to triple-negative breast cancer-related research.
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- CAS 番号: 471918-16-8
- 分子式: C29H39N9O4S
- 分子量:609.74
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
SNAP-Biotin (150 nM-1.5 mM) binds to the inactive SNAP-tag mutant SNAPC145A with measurable affinity[2].
SNAP-Biotin (1.5 μM; 30 min incubation, 11 h chase) labels parental H3.3-SNAP proteins in mouse embryonic stem cells[3].
SNAP-Biotin (1.5 μM; 30 min incubation, 14 h chase) labels parental H3.3-SNAP in mouse embryonic stem cells, where Mcm2-Pola1 and Pole3-Pole4 promote the transfer of parental H3.3-SNAP to the lagging strand and leading strand of DNA replication, respectively[3].
SNAP-Biotin (30 μM; 1 h) specifically labels the HCC1 fusion protein with a functional C-terminal SNAP tag in crude mitochondrial extracts from transgenic Arabidopsis hcc1-1 mutants[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 471918-16-8
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性状 Solid
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分子量 609.74
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分子式 C29H39N9O4S
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Color Off-white to light yellow
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SMILES
NC1=NC(OCC(C=C2)=CC=C2CNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=C5C(N=CN5)=N1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Stoops EH, et al. SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells. Methods in molecular biology (Clifton, N.J.). 2014;1174:171-82. [Content Brief]
[2]. Wilhelm J, et al. Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry. 2021 Aug 24;60(33):2560-2575. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
SNAP-Biotin 関連分類
HY-148958 関連分類
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