Fibronectin Adhesion-promoting Peptide TFA
Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide) is one of the heparin-binding amino acid sequences found in the carboxy-terminal heparin-binding domain of fibronectin. It promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates. Fibronectin Adhesion-promoting Peptide directly promotes the adhesion, spreading, and migration of endothelial cells by reacting with heparin binding domains of cells.
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- 화학식: C49H75F3N16O12
- 분자량:1137.22
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
GRGDS and Fibronectin Adhesion-promoting Peptide TFA (Heparin Binding Peptide TFA: WQPPRARI) micropatterns increase the endothelialisation of prosthetic materials in vitro. Fibronectin Adhesion-promoting Peptide also enhances haptotactic cell migration. The anti-apoptotic effects of fibronectin require the presence of the WQPPRARI sequence, which may be due to synergistic survival signals mediated by this sequence and by the RGD motif[1].
RDGS and Fibronectin Adhesion-promoting Peptide TFA promote cell adhesion through different activation pathways that induce different forms of adhesion which can be highlighted through the analysis of focal and fibrillar contacts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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분자량 1137.22
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화학식 C49H75F3N16O12
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Synonyms
Heparin Binding Peptide TFA
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Sequence
Trp-Gln-Pro-Pro-Arg-Ala-Arg-Ile
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Sequence Shortening
WQPPRARI
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Endothelial Tube Formation Assay
Endothelial tube formation assay evaluates the ability of endothelial cells to attach, migrate, align, and organize into capillary-like networks when cultured on gelled basement membrane extract or Matrigel; the readout is the morphology and quantity of tube-like networks, which reflects an in vitro endothelial morphogenesis step related to angiogenesis. Basement membrane extract/Matrigel provides laminin-rich extracellular matrix cues that support endothelial differentiation into capillary-like structures, but it can contain biologically active growth factors, so growth-factor-reduced matrix is preferred when testing defined angiogenic stimulators or inhibitors.
순도&문서
References
[1]. Hoesli CA, et al. A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces. Biomaterials. 2014 Jan;35(3):879-90. [Content Brief]
[2]. Chollet C, et al. Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress. Macromol Biosci. 2012 Dec;12(12):1648-59. [Content Brief]
[3]. Lei J, Murphy WL, et al. Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly. Bioconjug Chem. 2018 Apr 18;29(4):878-884. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fibronectin Adhesion-promoting Peptide TFA Related Classifications
HY-P0306A Related Classifications
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