PCL-2
PCL-2 is a reactive oxygen species (ROS)-responsive fluorescent probe. PCL-2 reacts with ROS to release 6-hydroxy-2-cyanobenzothiazole (HCBT), which then reacts with D-cysteine to form firefly luciferin in situ. PCL-2 must be used in conjunction with IETDC (HY-D3169) to detect the co-existence of hydrogen peroxide and caspase 8. PCL-2 is applicable to studies related to acute inflammation.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 1421277-81-7
- 화학식: C15H11BN2O3S
- 분자량:310.14
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
Biological Activity
제품 설명
In Vitro
PCL-2 (5 μM; 5, 20, 40, or 60 min) selectively produces a ca. 50-fold increase in bioluminescent signal in response to H2O2, but not other biologically relevant ROS, in a cell-free system[1].
PCL-2 (5 μM; 60 min) produces a linearly increasing bioluminescent signal in response to 1-100 μM H2O2 in a cell-free system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
PCL-2 (0.05 μmol; i.p.) produces a dose-dependent, 10-fold maximum bioluminescent turn-on signal in response to exogenous hydrogen peroxide in healthy FVB-luc+ mice, which is attenuated by a hydrogen peroxide scavenger[1].
PCL-2 (0.05 μmol; i.p.), in combination with IETDC, detects a ~2.7-fold increase in simultaneous endogenous hydrogen peroxide and caspase 8 activity during LPS (HY-D1056)-induced acute inflammation in female FVB-luc+ mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)[1]
-
Dosage:0.05 μmol (co-administered with 0.05 μmol D-cysteine)
-
Administration:intraperitoneal injection
-
Result:Produced a ~3.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~55% when co-administered with apocynin relative to signal from lipopolysaccharide alone.
-
Animal Model:FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)[1]
-
Dosage:0.05 μmol (co-administered with 0.05 μmol IETDC)
-
Administration:intraperitoneal injection
-
Result:Produced a ~2.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~30% when co-administered with ascorbic acid and z-VD(OMe)-OPh relative to signal from lipopolysaccharide alone.
Chemical Information
-
CAS No. 1421277-81-7
-
분자량 310.14
-
화학식 C15H11BN2O3S
-
SMILES
N#CC1=NC2=CC=C(C=C2S1)OCC3=CC=C(C=C3)B(O)O
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PCL-2 Related Classifications
HY-D3168 Related Classifications
Powered by Bioz
Keywords
- PCL-2
- 1421277-81-7
- PCL2
- PCL 2
- Fluorescent Dye
- Reactive Oxygen Species (ROS)
- reactive oxygen species
- caspase 8
- hydrogen peroxide
- FVB-luc+ mice
- lipopolysaccharide-induced acute inflammation
- apocynin
- ascorbic acid
- murine models of acute inflammation
- 6-hydroxy-2-cyanobenzothiazole
- PC3M-luc cells
- Inhibitor
- inhibitor
- inhibit