Lansiumamide B
Lansiumamide B is a cinnamamide alkaloid, Antifungal agent and Insecticide. Lansiumamide B is isolated from the seeds of Clausena lansium. Lansiumamide B inhibits the phosphorylation of p38 MAPK, and decreases the protein and mRNA levels of TNF-α. Lansiumamide B induces abnormal mycelial growth, cell content leakage and cell wall degradation in Rhizoctonia solani. Lansiumamide B causes the death of early 4th-instar larvae of Aedes albopictus. Lansiumamide B exhibits anticonvulsant effects in epileptic rat models. Lansiumamide B can be used in studies related to plant fungal diseases.
For research use only. We do not sell to patients.
- CAS No.: 121817-37-6
- Formula: C18H17NO
- Molecular Weight:263.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Lansiumamide B (100 μg/mL; 3-5 days) exhibits broad-spectrum in vitro antifungal activity against phytopathogenic fungi, acting against 10 species of phytopathogenic fungi, with the strongest activity against Rhizoctonia solani (EC50 = 2.99 μg/mL) and Botrytis cinerea (EC50 = 4.31 μg/mL)[1].
Lansiumamide B (100 µg/mL) potently inhibits the in vitro growth of 10 phytopathogenic fungi, with the highest inhibition rate (91.20%) against Thanatephorus cucumeris[3].
Lansiumamide B (5 μg/mL; 12 h) alters the transcriptome of Rhizoctonia solani, significantly upregulates genes related to polysaccharide hydrolysis, and enriches pathways associated with starch, sucrose and galactose metabolism. The transcriptome results are validated by qRT-PCR[1].
Lansiumamide B (5-50 μM; 30 min) dose-dependently inhibits histamine release from A23187-stimulated RBL-2H3 cells, reducing histamine release to 71.2% of that in the control group at 50 μM, with no cytotoxicity observed at this concentration[2].
Lansiumamide B (5-50 μM; 30 min) significantly reduces TNF-α secretion in A23187-stimulated RBL-2H3 cells, reaching only 47.5% of the control level at 50 μM, but exerts no significant inhibitory effect on COX-2 protein expression at concentrations up to 50 μM[2].
Lansiumamide B (5-50 μM; 30 min) does not significantly reduce the mRNA levels of COX-2, TNF-α or IL-6 in A23187-stimulated RBL-2H3 cells even at concentrations as high as 50 μM[2].
Lansiumamide B (5-50 μM; 30 min) reduces the level of phosphorylated p38 MAPK by 41.8% in A23187-stimulated RBL-2H3 cells at the concentration of 50 μM, but does not significantly alter the phosphorylation levels of PKCθ, IκBα or p65 NFκB even at the highest concentration of 50 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A23187-stimulated RBL-2H3 cells
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Concentration:5-50 μM
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Incubation Time:30 min pre-incubation, then 3 h stimulation
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Result:Subtly reduced COX-2, TNF-α, and IL-6 mRNA levels compared to A23187-stimulated controls, but none of these reductions were statistically significant.
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Cell Line:A23187-stimulated RBL-2H3 cells
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Concentration:5-50 μM
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Incubation Time:30 min pre-incubation, then 15 min stimulation
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Result:Caused a slight, non-statistically significant reduction in phosphorylated PKCθ relative intensity at 5 μM.
Reduced the relative intensity of phosphorylated p38 MAPK by 41.8% compared to A23187-stimulated controls at 50 μM, but this reduction was not statistically significant.
Had virtually no effect on the phosphorylation of IκBα or p65 NFκB.
In Vivo
Lansiumamide B (200-800 μg/mL; sprayed on both adaxial and abaxial leaf surfaces) exhibits concentration-dependent protective and curative effects against Botrytis cinerea on strawberry leaves[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:40-day-old plants[1]
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Dosage:200 μg/mL; 400 μg/mL; 800 μg/mL
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Administration:sprayed on adaxial and abaxial leaf surfaces
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Result:Showed 19.04% protective efficacy and 12.31% curative efficacy against Rhizoctonia solani at 200 μg/mL.
Showed 27.21% protective efficacy and 33.33% curative efficacy against Rhizoctonia solani at 400 μg/mL.
Reached 53.06% protective efficacy and 71.01% curative efficacy against Rhizoctonia solani at 800 μg/mL.
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Animal Model:40-day-old plants[1]
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Dosage:200 μg/mL; 400 μg/mL; 800 μg/mL
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Administration:sprayed on adaxial and abaxial leaf surfaces
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Result:Showed 7.09% protective efficacy and 31.37% curative efficacy against Botrytis cinerea at 200 μg/mL.
Showed 41.54% protective efficacy and 48.82% curative efficacy against Botrytis cinerea at 400 μg/mL.
Reached 61.39% protective efficacy and 67.95% curative efficacy against Botrytis cinerea at 800 μg/mL.
Chemical Information
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CAS No. 121817-37-6
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Molecular Weight 263.33
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Formula C18H17NO
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SMILES
CN(/C=C\C1=CC=CC=C1)C(/C=C/C2=CC=CC=C2)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Kinase activity and phosphorylation assays
Kinase activity assays measure the ability of kinases to transfer phosphate groups from ATP to specific substrates, while phosphorylation assays detect the presence and levels of phosphorylated proteins. Common methods include radiolabeled ATP incorporation (e. g. ,), ADP release detection via bioluminescence (e. g. ,[3]), enzyme-linked immunosorbent assays (ELISA) for phospho-specific epitopes (e. g. ,[6]), and microtiter-based formats for high-throughput screening (e. g. ,[8]). The ADP-Glo assay quantifies kinase activity by measuring ADP produced during phosphorylation using a luciferase-based system. Radiometric assays involve autoradiography or scintillation counting after incorporation of 32P-labeled ATP into substrate proteins. ELISA-based approaches rely on phospho-specific antibodies to detect activated kinases in cell lysates or purified samples.
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Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Filamentous Fungal Mold Culture and Sporulation
Filamentous fungal mold culture and sporulation assays grow hyphae under defined nutritional and environmental conditions until asexual spores, commonly conidia, are produced; the main readouts are colony growth, sporulation onset, conidial yield, conidial morphology, viability, and, when relevant, downstream infectivity or stress phenotype.
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Protocol for Kinase activity and phosphorylation assays
Kinase activity assays measure transfer of phosphate from ATP to a protein or peptide substrate, generating phosphorylated substrate, ADP, or incorporated radiolabeled phosphate as the readout; phosphorylation assays measure site-specific phosphorylation in cells or tissues as a proxy for kinase-pathway activation, inhibition, or substrate regulation. Phosphorylation can be detected by phospho-specific Western blot, immunoprecipitation kinase assay, phospho-immunofluorescence, phospho-flow cytometry, luminescent ADP detection, radiolabeled ATP incorporation, or reporter-based pathway assays, and these readouts can be applied to cancer cells, primary neurons, mouse tumors, organoids, inflammatory macrophages, ferroptosis studies, and mitophagy studies when the kinase target is biologically relevant.
Purity & Documentation
References
[2]. Matsui T, et al. Lansiumamide B and SB-204900 isolated from Clausena lansium inhibit histamine and TNF-α release from RBL-2H3 cells. Inflamm Res. 2013 Mar;62(3):333-41. [Content Brief]
[3]. Xu H, et al. Synthesis and Fungicidal Activity of Lansiumamide A and B and Their Derivatives. Molecules (Basel, Switzerland). 2018 Jun 21;23(7):1499. [Content Brief]
[4]. Han Y, et al. Larvicidal activity of lansiumamide B from the seeds of Clausena lansium against Aedes albopictus (Diptera: Culicidae). Parasitology research. 2013 Feb;112(2):511-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Lansiumamide B Related Classifications
HY-N20748 Related Classifications
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