Lansiumamide B and SB-204900 isolated from Clausena lansium inhibit histamine and TNF-α release from RBL-2H3 cells

  • Inflamm Res. 2013 Mar;62(3):333-41. doi: 10.1007/s00011-012-0586-8.
Takuya Matsui  1 ,  Chihiro Ito ,  Hiroshi Furukawa ,  Tadashi Okada ,  Masataka Itoigawa
Affiliations
  • 1. Faculty of Pharmacy, Meijo University, Tempaku-ku, Nagoya, Aichi, Japan. [email protected]
PMID: 23324895 DOI: 10.1007/s00011-012-0586-8
Abstract

Aims and objective: Mast cells play a central role in allergic and chronic inflammation. Extracts from Clausena lansium (Lour.) Skeels (Rutaceae) possess many pharmacological effects including anti-inflammatory, anti-oxidant, anti-cancer, and anti-trichomonal activities. In addition, the leaves and fruit are used in Chinese folk medicine. We have isolated and identified four known cinnamamides from this plant: lansiumamide C, lansamide I, lansiumamide B, and SB-204900. However, the biological activities of these compounds are not yet understood. The purpose of this paper is to clarify the pharmacological effects of these compounds on mast cells.

Methods: We measured inflammatory molecules in A23187-stimulated rat basophilic leukemia cells (RBL-2H3) treated with these compounds using HPLC, ELISA, and immunoblotting methods. In addition, some signaling molecules were investigated by immunoblotting.

Results: Lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release. Furthermore, lansiumamide B- and SB-204900-treated cells also reduced the protein and/or mRNA levels of TNF-α. SB-204900 markedly suppressed the phosphorylation of p38 MAPK.

Conclusion: Our findings suggest that lansiumamide B and SB-204900 attenuate mast-cell-induced inflammation.

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