Leishman's stain
Based on 1 Customer Validation
Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens).
For research use only. We do not sell to patients.
- CAS No.: 12627-53-1
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare stock solution of Sorensen's buffer: Solution A: Na2HPO42H2O (9.5 g/L); Solution B: KH2PO4 (9.1 g/L).
2. Cover the smear with Leishman stain for 2 min.
3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min.
4. Wash off with running water and dry the smear (5 min).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 12627-53-1
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Appearance Solid
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Color Green to dark green
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SMILES
[Leishman's stain]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (264 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)