Leucine dehydrogenase, Microorganism
Based on 1 Customer Validation
Leucine dehydrogenase, Microorganism (EC 1.4.1.9) can be purified from Bacillus spheroides. Leucine dehydrogenase catalyzed the oxidative deamination of L-leucine, L-valine, L-isoleucine, L-norvaline, L-alpha-aminobutyrate, and L-norleucine, and the reductive amination of their keto analogues.
For research use only. We do not sell to patients.
- CAS No.: 9082-71-7
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Product Information
Molecular weight: 43 kDa (SDS-PAGE)
Isoelectric point: 6.6
Km value: 2.6×10-4 M (NAD ), 2.0×10-3 M(L-Leucine) , 6.8×10-4 M(α-Ketoisocaproate), 4.2×10-2 M (NH Cl), 2.3×10-4 M (NADH)
pH stability: 6.0-11.0 (25°C, 15 hr)
Optimum temperature: 55-60°C
Inhibitor: Hg2+
Instructions
Dissolved in water (1 mg/mL), or in buffer according to the specific experimental reference.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1.4.1.9
≥25 U/mg protein
One unit is defined as the amount of enzyme that will convert 1.0 μmole of L-leucine to αketoisocaproate per min at pH 10.5 at 37°C.
Chemical Information
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CAS No. 9082-71-7
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Appearance Solid
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Color White to light yellow
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SMILES
[Leucine dehydrogenase, Microorganism]
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Synonyms
LDH, EC 1.4.1.9
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Leucine dehydrogenase, Microorganism Related Classifications
HY-P2768 Related Classifications
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