Liensinine Diperchlorate

Name: Liensinine Diperchlorate
Brand: MedChemExpress
SKU: HY-N0485
Rating: 5.0 (20 reviews)

Cat. No.: HY-N0485 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Liensinine Diperchlorate is a major isoquinoline alkaloid, extracted from the seed embryo of Nelumbo nucifera Gaertn. Liensinine Diperchlorate inhibits late-stage autophagy/mitophagy through blocking autophagosome-lysosome fusion. Liensinine Diperchlorate has a wide range of biological activities, including anti-arrhythmias, anti-hypertension, anti-pulmonary fibrosis, relaxation on vascular smooth muscle, etc.

For research use only. We do not sell to patients.

CAS No. : 5088-90-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 20 publication(s) in Google Scholar

Other Forms of Liensinine Diperchlorate:

Liensinine In-stock
Liensinine perchlorate In-stock

20 Publications Citing Use of MCE Liensinine Diperchlorate

Cell Proliferation/Viability Assay

Flow Cytometry

Cell Imaging/Staining

RT-PCR

: Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul 31:86:103793. [Abstract]; The viability of HBE cells treated with different concentration of Liensinine (LIE) (10, 20, 40, 60, 80 μM) measured by CCK8 assay.

: Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul 31:86:103793. [Abstract]; Liensinine (LIE) (40 μM) treatment effectively suppressed autophagy by Western blot.

: Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul 31:86:103793. [Abstract]; Liensinine (LIE) (40 μM) improved mitochondrial damage induced by ARSK knockdown, as evidenced by the reductions in intracellular and mitochondrial ROS levels.

: Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul 31:86:103793. [Abstract]; SA-β-gal staining results demonstrated that Liensinine (LIE) (40 μM) treatment obviously decreased the percentage of positive cells.

: Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul 31:86:103793. [Abstract]; The expressions of IL-6, IL-8 and IL-1β were both relived in HBE cells by Liensinine (LIE) (40 μM).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Clinical Trial

Molecular Weight

811.66

Formula

C₃₇H₄₄Cl₂N₂O₁₄

CAS No.

5088-90-4

Appearance

Solid

Color

White to off-white

SMILES

O=Cl(=O)(O)=O.CN1[C@@H](C2=CC(OC3=CC(C[C@@H]4C5=CC(OC)=C(OC)C=C5CCN4C)=CC=C3O)=C(OC)C=C2CC1)CC6=CC=C(O)C=C6.O=Cl(=O)(O)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (77.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2320 mL	6.1602 mL	12.3204 mL
5 mM	0.2464 mL	1.2320 mL	2.4641 mL
10 mM	0.1232 mL	0.6160 mL	1.2320 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (253 KB) HNMR (324 KB) RP-HPLC (245 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Zhou J, et al. A novel autophagy/mitophagy inhibitor liensinine sensitizes breast cancer cells to chemotherapy through DNM1L-mediated mitochondrial fission. Autophagy. 2015;11(8):1259-79. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2320 mL	6.1602 mL	12.3204 mL	30.8011 mL
	5 mM	0.2464 mL	1.2320 mL	2.4641 mL	6.1602 mL
	10 mM	0.1232 mL	0.6160 mL	1.2320 mL	3.0801 mL
	15 mM	0.0821 mL	0.4107 mL	0.8214 mL	2.0534 mL
	20 mM	0.0616 mL	0.3080 mL	0.6160 mL	1.5401 mL
	25 mM	0.0493 mL	0.2464 mL	0.4928 mL	1.2320 mL
	30 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0267 mL
	40 mM	0.0308 mL	0.1540 mL	0.3080 mL	0.7700 mL
	50 mM	0.0246 mL	0.1232 mL	0.2464 mL	0.6160 mL
	60 mM	0.0205 mL	0.1027 mL	0.2053 mL	0.5134 mL