Cat. No.: HY-N0485

Liensinine Diperchlorate

21 Cited Publications

Customer Review

Based on 21 publication(s) in Google Scholar

Liensinine Diperchlorate is a major isoquinoline alkaloid, extracted from the seed embryo of Nelumbo nucifera Gaertn. Liensinine Diperchlorate inhibits late-stage autophagy/mitophagy through blocking autophagosome-lysosome fusion. Liensinine Diperchlorate has a wide range of biological activities, including anti-arrhythmias, anti-hypertension, anti-pulmonary fibrosis, relaxation on vascular smooth muscle, etc.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 5088-90-4
Formula: C₃₇H₄₄Cl₂N₂O₁₄
Molecular Weight:811.66

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Liensinine Diperchlorate Chemical Structure

CAS No.: 5088-90-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Clinical Trial
Chemical Information
Publications (21)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 5088-90-4
Appearance Solid
Molecular Weight 811.66
Formula C₃₇H₄₄Cl₂N₂O₁₄
Color White to off-white
SMILES

O=Cl(=O)(O)=O.CN1[C@@H](C2=CC(OC3=CC(C[C@@H]4C5=CC(OC)=C(OC)C=C5CCN4C)=CC=C3O)=C(OC)C=C2CC1)CC6=CC=C(O)C=C6.O=Cl(=O)(O)=O
Structure Classification
- Alkaloids
- Piperidine Alkaloids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (21)

All Cell Proliferation/Viability AssayWBFlow CytometryCell Imaging/StainingRT-PCR

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Journal Impact Factor

Signal Transduct Target Ther

Disruption of heme homeostasis by nuclear receptor Nur77 induces pyroptosis through granzyme B-dependent GSDMC cleavage. [Abstract]

2025 Dec 17;10(1):413. PMID: 41407678
Redox Biol

Arylsulfatase K attenuates airway epithelial cell senescence in COPD by regulating parkin-mediated mitophagy. [Abstract]

2025 Oct:86:103793. PMID: 40763656

Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

The viability of HBE cells treated with different concentration of Liensinine (LIE) (10, 20, 40, 60, 80 μM) measured by CCK8 assay.

Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

Liensinine (LIE) (40 μM) treatment effectively suppressed autophagy by Western blot.

Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

Liensinine (LIE) (40 μM) improved mitochondrial damage induced by ARSK knockdown, as evidenced by the reductions in intracellular and mitochondrial ROS levels.

Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

SA-β-gal staining results demonstrated that Liensinine (LIE) (40 μM) treatment obviously decreased the percentage of positive cells.

Liensinine Diperchlorate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

The expressions of IL-6, IL-8 and IL-1β were both relived in HBE cells by Liensinine (LIE) (40 μM).
Cell Death Dis

Crotonylated BEX2 interacts with NDP52 and enhances mitophagy to modulate chemotherapeutic agent-induced apoptosis in non-small-cell lung cancer cells. [Abstract]

2023 Sep 30;14(9):645. PMID: 37777549
Int J Biol Sci

Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation. [Abstract]

2022 Aug 8;18(13):5168-5184. PMID: 35982894
Anim Nutr

Caffeic acid phenethyl ester ameliorates high-fat diet-induced muscle textural deterioration in grass carp (Ctenopharyngodon idellus) by modulating adipose-muscle crosstalk via myostatin-taz signaling. [Abstract]

2026 Feb 6:25:160-174. PMID: 41938616
Chin Med

Targeting G-protein-coupled receptors and gut microbiota: Ge-Lian Qi-Shen decoction elevates GLP-1 to combat non-alcoholic fatty liver disease. [Abstract]

2026 Jan 24;21(1):51. PMID: 41580820
J Ethnopharmacol

Integrating UHPLC-Q-TOF-MS/MS, network pharmacology, bioinformatics and experimental validation to uncover the anti-cancer mechanisms of TiaoPi AnChang decoction in colorectal cancer. [Abstract]

2024 Nov 15:334:118576. PMID: 39002822
Life Sci

Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage. [Abstract]

2023 Jun 1:322:121653. PMID: 37011875
Int J Mol Sci

Liensinine Prevents Acute Myocardial Ischemic Injury via Inhibiting the Inflammation Response Mediated by the Wnt/β-Catenin Signaling Pathway. [Abstract]

2025 May 10;26(10):4566. PMID: 40429711
Molecules

Molecular Mechanisms Underlying the Anti-Tumor Activity of Lotus-Derived Alkaloids in Breast Cancer. [Abstract]

2026 Mar 12;31(6):947. PMID: 41900047
J Mol Med (Berl)

Liensinine can improve vascular remodeling in hypertension through the ferroptosis-related TLR4 inflammatory pathway. [Abstract]

2025 Dec 26;104(1):13. PMID: 41452356
J Biol Chem

Activation of Nemo-like Kinase in Diamond Blackfan Anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis. [Abstract]

2024 Aug;300(8):107542. PMID: 38992436
Adipocyte

The combination of nuclear receptor NR1D1 and ULK1 promotes mitophagy in adipocytes to ameliorate obesity. [Abstract]

2022 Dec;11(1):202-212. PMID: 35410572
Tissue Cell

Liensinine induces autophagy and apoptosis in hepatocellular carcinoma via reactive oxygen species-mediated inhibition of the PI3K/AKT/mTOR pathway. [Abstract]

2026 Jun 12:103:103696. PMID: 42314534
Genes Nutr

Liensinine alleviates type 2 diabetes mellitus through modulating the pancreatic β cell function and gut microbiota. [Abstract]

2025 Dec 24;20(1):29. PMID: 41444518
J Int Med Res

RNA sequencing-based evaluation of the mechanisms underlying the liensinine-mediated inhibition of hypopharyngeal cancer proliferation. [Abstract]

2026 Feb;54(2):3000605261419604. PMID: 41698653
Lett Drug Des Discov

2025 Dec 2.
SSRN

2023 Apr 18.
Research Square Print

2023 Mar 24.
J Oncol

Liensinine Inhibits Cell Growth and Blocks Autophagic Flux in Nonsmall-Cell Lung Cancer. [Abstract]

2022 Jul 1:2022:1533779. PMID: 35813859
Oxid Med Cell Longev

Liensinine Inhibits Osteosarcoma Growth by ROS-Mediated Suppression of the JAK2/STAT3 Signaling Pathway. [Abstract]

2022 Jan 25:2022:8245614. PMID: 35116094

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (77.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2320 mL

6.1602 mL

12.3204 mL

5 mM

0.2464 mL

1.2320 mL

2.4641 mL

10 mM

0.1232 mL

0.6160 mL

1.2320 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (2.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhou J, et al. A novel autophagy/mitophagy inhibitor liensinine sensitizes breast cancer cells to chemotherapy through DNM1L-mediated mitochondrial fission. Autophagy. 2015;11(8):1259-79. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2320 mL	6.1602 mL	12.3204 mL	30.8011 mL
	5 mM	0.2464 mL	1.2320 mL	2.4641 mL	6.1602 mL
	10 mM	0.1232 mL	0.6160 mL	1.2320 mL	3.0801 mL
	15 mM	0.0821 mL	0.4107 mL	0.8214 mL	2.0534 mL
	20 mM	0.0616 mL	0.3080 mL	0.6160 mL	1.5401 mL
	25 mM	0.0493 mL	0.2464 mL	0.4928 mL	1.2320 mL
	30 mM	0.0411 mL	0.2053 mL	0.4107 mL	1.0267 mL
	40 mM	0.0308 mL	0.1540 mL	0.3080 mL	0.7700 mL
	50 mM	0.0246 mL	0.1232 mL	0.2464 mL	0.6160 mL
	60 mM	0.0205 mL	0.1027 mL	0.2053 mL	0.5134 mL

Keywords

Liensinine Diperchlorate

21 Cited Publications

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Liensinine Diperchlorate

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