Art. -Nr.: HY-115576

P62-mediated mitophagy inducer

Name: P62-mediated mitophagy inducer
Brand: MedChemExpress
SKU: HY-115576
Price: 80.00 USD
Availability: InStock
Rating: 5 (6 reviews)

6 Cited Publications

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Based on 6 publication(s) in Google Scholar

P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator. P62-mediated mitophagy inducer activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential and remains active in cells lacking a fully functional PINK1/Parkin pathway. P62-mediated mitophagy inducer serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential.

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Reinheit : 99.54%
CAS. Nr.: 1809031-84-2
Formel: C₁₄H₉IN₄O₂
Molecular Weight:392.15

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 2 years , -20°C, 1 year

P62-mediated mitophagy inducer Chemical Structure

CAS. Nr.: 1809031-84-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (6)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll (7)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

mitophagy^[1].

In Vitro

P62-mediated mitophagy inducer (10 μM; 0, 1, 3, 6, 24 h) stabilizes Nrf2 and (10 μM; 9 h) upregulates P62 expression activating mitophagy^[1].
P62-mediated mitophagy (10 μM; 24 h) acts downstream of the PINK1/Parkin signaling pathway in MEFs^[1].
P62-mediated mitophagy inducer positively affects mitochondrial poly-ubiquitination and coupling^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

RT-PCR^[1]

View Protocol

Cell Line:

MEFs
Concentration:

10 µM
Incubation Time:

9 h
Result:

Significantly increased p62 mRNA levels.

Immunofluorescence^[1]

View Protocol

Cell Line:

MEFs
Concentration:

10 µM
Incubation Time:

24 h
Result:

Demonstrated Parkin-independent induction of mitochondrial recruitment of P62.

Western Blot Analysis^[1]

View Protocol

Cell Line:

MEFs
Concentration:

10 µM
Incubation Time:

0, 1, 3, 6, 24 h
Result:

Exhibited maximum Nrf2 levels after 6 h and remained elevated at 24 h.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 1809031-84-2
Appearance Solid
Molecular Weight 392.15
Formel C₁₄H₉IN₄O₂
Color Light yellow to yellow
SMILES

O=[N+](C1=CC(C2=CN(C3=CC=CC(I)=C3)N=N2)=CC=C1)[O-]
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayWBIFRT-PCR

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Journal Impact Factor

Autophagy

MANF facilitates breast cancer cell survival under glucose-starvation conditions via prkn-mediated mitophagy regulation. [Abstract]

2025 Jan;21(1):80-101. PMID: 39147386

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jan;21(1):80-101. [Abstract]

Both pharmacological mitophagy inhibition (baf A1 and mdivi-1) and genetic mitophagy inhibition (DNM1L-KD) reduced and mitophagy agonist PMI (P62-mediated mitophagy inducer, 0.1-10 μM) increased BC cell viability under glucose-starvation conditions (n = 3).
Genes Dis

Mitophagy deficiency activates stimulator of interferon genes activation and aggravates pathogenetic cardiac remodeling. [Abstract]

2023 Sep 2;11(6):101074. PMID: 39281830

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Genes Dis. 2023 Sep 2;11(6):101074. [Abstract]

Protein levels of Parkin in CFs exposed to Ang II (100 nM) for 24 h with siRNA-Parkin or PMI (P62-mediated mitophagy inducer).

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Genes Dis. 2023 Sep 2;11(6):101074. [Abstract]

CF mitophagy was confirmed by the co-location of mito-tracker green and lyso-tracker red after exposure to Ang II for 24 h with siRNA-Parkin or PMI (P62-mediated mitophagy inducer).

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Genes Dis. 2023 Sep 2;11(6):101074. [Abstract]

Protein levels and quantification of STING, p-IRF3, and IRF3 in CFs exposed to Ang II with siRNA-Parkin or PMI (P62-mediated mitophagy inducer).

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Genes Dis. 2023 Sep 2;11(6):101074. [Abstract]

Representative immunofluorescence staining and quantitative analysis of α-SMA in CFs exposed to Ang II with siRNA-Parkin or PMI (P62-mediated mitophagy inducer).

P62-mediated mitophagy inducer purchased from MedChemExpress. Usage Cited in: Genes Dis. 2023 Sep 2;11(6):101074. [Abstract]

CF cytosolic mtDNA levels were measured with RT-PCR after exposure to Ang II for 24 h with siRNA-Parkin or PMI (P62-mediated mitophagy inducer).
Free Radic Biol Med

DRP1-mediated mitophagy facilitates oral squamous cell carcinoma tumor cell survival under glucose restriction. [Abstract]

2026 Jun 27:254:391-404. PMID: 42364552
Radiother Oncol

Mitophagy induction improves salivary gland stem/progenitor cell function by reducing senescence after irradiation. [Abstract]

2024 Jan:190:110028. PMID: 38007043
Cancer Res Commun

Combination of a New Oral Demethylating Agent, OR2100, and Venetoclax for Treatment of Acute Myeloid Leukemia. [Abstract]

2023 Feb 21;3(2):297-308. PMID: 36860654
bioRxiv

LOXL2 Deletion Triggers TMJ Osteoarthritis While Overexpression Protects Against NF-κβ-Induced Chondrocyte Apoptosis. [Abstract]

2025 May 13:2025.05.12.653519. PMID: 40463059

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 8.33 mg/mL (21.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5500 mL

12.7502 mL

25.5004 mL

5 mM

0.5100 mL

2.5500 mL

5.1001 mL

10 mM

0.2550 mL

1.2750 mL

2.5500 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (3.19 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (3.19 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

All Signal Pathway Related Cell Biology

Mitochondrial membrane-potential fluorescent assay
Mitochondrial membrane potential fluorescent assays estimate ΔΨm in living cells using lipophilic cationic dyes such as TMRM, TMRE, rhodamine 123, and JC-1, which accumulate in mitochondria according to membrane polarization; loss of signal after FCCP or CCCP treatment is interpreted as mitochondrial depolarization. TMRM/TMRE and rhodamine 123 are commonly used for semi-quantitative live-cell microscopy or flow cytometry, while JC-1 can report a shift from red aggregate fluorescence to green monomer fluorescence during depolarization; interpretation requires controls because dye concentration, quenching mode, cell type, dye efflux, and mitochondrial mass can affect fluorescence independently of ΔΨm.
Fluorescent plasma-membrane potential dye assay
Fluorescent plasma-membrane potential dye assays measure changes in cell membrane potential using voltage-sensitive dyes whose fluorescence changes when cells depolarize or hyperpolarize. Anionic bis-oxonol dyes such as DiBAC4(3) enter depolarized cells more readily and show increased fluorescence after intracellular binding, while hyperpolarization reduces dye accumulation and fluorescence. FMP/FLIPR membrane-potential dyes are used for faster, homogeneous microplate assays of ion-channel or receptor-mediated membrane-potential changes.
Autophagy
Autophagy is a process in which eukaryotic cells use lysosomes to degrade their own cytoplasmic proteins and damaged organelles under the regulation of autophagy related gene (Atg). Microtubule-associated proteins light chain 3 (LC3) is recognized as autophagy marker, which transfers from cytoplasmic LC3 (LC3-I) to membrane type (LC3-II). LC3-II/I ratio could be detected by Western Blot and fluorescence microscopy.
Mitochondrial membrane-potential and mitochondrial mass staining
Mitochondrial membrane potential staining measures the electrochemical polarization across the mitochondrial inner membrane in live cells using lipophilic cationic fluorescent probes; early rhodamine-based work showed that selective mitochondrial dye accumulation is lost when the mitochondrial transmembrane potential is dissipated. JC-1 reports mitochondrial polarization by shifting from green monomer fluorescence to red J-aggregate fluorescence as dye concentration increases within energized mitochondria; therefore, the red/green fluorescence ratio is used as a relative readout of mitochondrial membrane potential. TMRE or TMRM staining provides a single-channel relative readout because these cationic rhodamine esters accumulate in polarized mitochondria, and lower fluorescence indicates reduced mitochondrial polarization when acquisition and dye-loading conditions are controlled. Mitochondrial mass staining is commonly performed with MitoTracker Green FM or related MitoTracker dyes as
Lysosome and acidic-vesicle live-cell staining
Lysosome and acidic-vesicle live-cell staining detects acidic intracellular compartments by using membrane-permeant acidotropic probes that accumulate in low-pH vesicles, including lysosomes, late endosomes, autolysosomes, and acidic phagosomes. LysoTracker staining is commonly used as an intensity-based readout of acidic lysosomal compartment abundance or enlargement, while acridine orange produces green fluorescence in less concentrated compartments and red fluorescence after concentration-dependent accumulation in acidic vesicular organelles. Loss or reduction of acridine-orange red signal can be used as a readout of lysosomal membrane permeabilization or reduced acidic-vesicle integrity. This protocol is designed for live cultured cells and can be adapted for fluorescence microscopy, high-content imaging, plate-reader readout, or flow cytometry when the selected literature supports the readout. Because these dyes report acidotropic accumulation rather than lysosome identity alone,
Mitophagy Solutions
Mitophagy is the selective autophagic degradation of mitochondria and functions as a mitochondrial quality-control pathway that removes damaged, depolarized, excess, or developmentally programmed mitochondria. The pathway links mitochondrial damage recognition, autophagosome recruitment, lysosomal delivery, and mitochondrial turnover to phenotypes such as mitochondrial homeostasis, oxidative-stress control, metabolic remodeling, differentiation, and neurodegeneration-related mitochondrial fidelity. The best-characterized damage-induced pathway is the PINK1-Parkin axis. Parkin is recruited selectively to impaired mitochondria and promotes their autophagic elimination, while mitochondrial depolarization stabilizes PINK1 on damaged mitochondria, recruits Parkin, and activates Parkin-dependent mitophagy. PINK1 also phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity, and PINK1-driven ubiquitin phosphorylation creates a feed-forward signal for recruiting autophagy machi
Macroautophagy Solutions
Macroautophagy is a conserved lysosome-dependent degradation pathway in which cytoplasmic material is sequestered into double-membrane autophagosomes and delivered to lysosomes for degradation and recycling. The pathway supports cellular homeostasis during nutrient limitation, organelle stress, protein-aggregate accumulation, infection, differentiation, and tissue remodeling by coupling cargo sequestration, autophagosome maturation, lysosomal fusion, and degradation of cargo-derived macromolecules. The core molecular sequence includes initiation by nutrient- and stress-regulated autophagy machinery, autophagosome nucleation, LC3/ATG8-family conjugation to autophagosomal membranes, cargo selection through receptors such as SQSTM1/p62, autophagosome-lysosome fusion, and lysosomal degradation. LC3 was identified as a mammalian homolog of yeast Atg8 that localizes to autophagosomal membranes after processing, and p62/SQSTM1 was shown to connect ubiquitinated cargo with autophagic degradati

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. East DA, et al. PMI: a ΔΨm independent pharmacological regulator of mitophagy. Chem Biol. 2014 Nov 20;21(11):1585-96. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5500 mL	12.7502 mL	25.5004 mL	63.7511 mL
	5 mM	0.5100 mL	2.5500 mL	5.1001 mL	12.7502 mL
	10 mM	0.2550 mL	1.2750 mL	2.5500 mL	6.3751 mL
	15 mM	0.1700 mL	0.8500 mL	1.7000 mL	4.2501 mL
	20 mM	0.1275 mL	0.6375 mL	1.2750 mL	3.1876 mL

Bioz

Keywords

P62-mediated mitophagy inducer

6 Cited Publications

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P62-mediated mitophagy inducer

Publications Citing Use of MedChemExpress (MCE) P62-mediated mitophagy inducer