Cat. No.: HY-N3376

Liriodenine

Name: Liriodenine
Brand: MedChemExpress
SKU: HY-N3376
Price: 700.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Spermatheridine; VLT045)

고객리뷰

Based on 1 Customer Validation

Liriodenine (Spermatheridine; VLT045) is an aporphine alkaloid isolated from the plant?Mitrephora sirikitiae?and has anti-cancer activities. Liriodenine induces cell apoptosis, activates the intrinsic pathway by induction of caspase-3 and caspase-9.

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Purity : 97%
CAS No.: 475-75-2
화학식: C₁₇H₉NO₃
분자량:275.26

보관:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Liriodenine Chemical Structure

CAS No.: 475-75-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-122930
Lysicamine

Antibacterial Agent

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
Chemical Information
Protocol (4)
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Liriodenine (Spermatheridine; VLT045) is an aporphine alkaloid isolated from the plant?Mitrephora sirikitiae?and has anti-cancer activities^[1]. Liriodenine induces cell apoptosis, activates the intrinsic pathway by induction of caspase-3 and caspase-9^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	0.72 μg/mL Compound: 1	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 2292689]
B16-F10	IC₅₀	>10 μM Compound: 20	Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay	[PMID: 27300257]
BV-2	IC₅₀	5 μM Compound: 13	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay	[PMID: 33822610]
HCT-8	ED₅₀	0.7 μg/mL Compound: 1	Cytotoxicity against human HCT8 cells Cytotoxicity against human HCT8 cells	[PMID: 2292689]
HepG2	IC₅₀	8.3 μM Compound: 20	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay	[PMID: 27300257]
HL-60	IC₅₀	5.5 μM Compound: 20	Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay	[PMID: 27300257]
HT-29	IC₅₀	>10 μM Compound: 6	Cytotoxicity against human HT-29 cells assessed as inhibition of cell proliferation after 3 days by SRB assay Cytotoxicity against human HT-29 cells assessed as inhibition of cell proliferation after 3 days by SRB assay	[PMID: 23327794]
K562	IC₅₀	5 μM Compound: 20	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay	[PMID: 27300257]
KB	ED₅₀	1 μg/mL Compound: 1	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 2292689]
KB	ED₅₀	3.1 μg/mL Compound: liriodenine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	10.1021/np50019a011
KB	IC₅₀	5 μM Compound: 9	Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay	[PMID: 19874044]
L1210	ED₅₀	2.33 μg/mL Compound: 1	Cytotoxicity against mouse L1210 cells Cytotoxicity against mouse L1210 cells	[PMID: 2292689]
MCF7	IC₅₀	5 μM Compound: 9	Cytotoxicity against human MCF7 cells after 3 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells after 3 days by sulforhodamine B assay	[PMID: 19874044]
NCI-H460	IC₅₀	5 μM Compound: 9	Cytotoxicity against human NCI-H460 cells after 3 days by sulforhodamine B assay Cytotoxicity against human NCI-H460 cells after 3 days by sulforhodamine B assay	[PMID: 19874044]
P388	ED₅₀	0.57 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 2292689]
PBMC	IC₅₀	34.8 μM Compound: 20	Cytotoxicity against human PBMC cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay Cytotoxicity against human PBMC cells assessed as reduction in cell viability incubated for 72 hrs by Alamar blue assay	[PMID: 27300257]
RAW264.7	IC₅₀	1.39 μM Compound: 129	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay	[PMID: 33454546]
RAW264.7	IC₅₀	10.5 μM Compound: 10	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 33939429]
RAW264.7	IC₅₀	34.7 μM Compound: 6	Inhibition of LPS-stimulated nitric oxide production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay Inhibition of LPS-stimulated nitric oxide production in mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay	[PMID: 32247751]
SF-268	IC₅₀	5 μM Compound: 9	Cytotoxicity against human SF268 cells after 3 days by sulforhodamine B assay Cytotoxicity against human SF268 cells after 3 days by sulforhodamine B assay	[PMID: 19874044]
Vero	CC₅₀	48 μM Compound: 16	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 9584402]

In Vitro

Liriodenine is against P-388, KB, HT-29, MCF-7, A549, ASK and Hek-293 cells with IC₅₀ values of 9.60 μM, 11.02 μM, 10.62 μM, 9.20 μM and 8.07μM, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 475-75-2
Appearance Solid
분자량 275.26
화학식 C₁₇H₉NO₃
Color Light yellow to yellow
SMILES

O=C1C2=CC=CC=C2C3=C4OCOC4=CC5=CC=NC1=C53
Synonyms

Spermatheridine; VLT045
Structure Classification
- Alkaloids
- Pyridine Alkaloids
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Protocol

All Cell Biology Signal Pathway Related

Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (421 KB)
- English - EN (421 KB)
- Français - FR (421 KB)
- Deutsch - DE (421 KB)
- Norwegian - NO (421 KB)
- Español - ES (421 KB)
- Swedish - SV (421 KB)
- Italian - IT (421 KB)
- Korean - KR (421 KB)
- Portuguese - PT (421 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Natthinee Anantachoke, et al. Cytotoxic Compounds From the Leaves and Stems of the Endemic Thai Plant Mitrephora sirikitiae. Pharm Biol [Content Brief]

[2]. Noraziah Nordin, et al. Liriodenine, an Aporphine Alkaloid From Enicosanthellum Pulchrum, Inhibits Proliferation of Human Ovarian Cancer Cells Through Induction of Apoptosis via the Mitochondrial Signaling Pathway and Blocking Cell Cycle Progression. Drug Des Devel Ther [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Liriodenine