MB-Buf
MB-Buf is a conjugation of methylene blue (HY-14536) and bufalin (HY-N0877) . MB-Buf can targeting degrade GPX4 upon light activation. MB-Buf can induce cell apoptosis, ferroptosis, and ROS production. MB-Buf can be used for the research of cancer, such as breast cancer.
For research use only. We do not sell to patients.
- Formula: C47H55N3O5S
- Molecular Weight:774.02
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
GPX4 |
MB-Buf (5 μM, 660 nm laser irradiation for 0-5 min) induces singlet oxygen, superoxide anion and hydroxyl radical generation[1].
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) exhibits the strongest inhibitory activity against MCF-7, MDA-MB-231 and 4T1 cells with IC50 values of 28.74, 36.82 and 64.41 nM, respectively[1].
MB-Buf (0.1-1 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) induces ROS production and ferroptosis in MCF-7 cells[1].
MB-Buf (10-100 nM, 6 h pretreatment + 660 nm laser irradiation for 2-5 min) degrades GPX4 in MCF-7 cells[1].
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) inhibits migration in MCF-7 cells[1].
MB-Buf (0.5 μM, 6 h pretreatment with 660 nm laser irradiation for 5 min) induces apoptosis in MCF-7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MCF-7 cells
-
Concentration:10, 50 and 100 nM
-
Incubation Time:6 h pretreatment + 660 nm laser irradiation for 2-5 min
-
Result:Reduced GPX4 levels.
-
Cell Line:MCF-7 cells
-
Concentration:0.1, 0.5 and 1 μM
-
Incubation Time:6 h pretreatment with 660 nm laser irradiation for 5 min
-
Result:Increased ROS production.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:4 T1-Luci xenograft mice models[1]
-
Dosage:1 mg/kg
-
Administration:Intravenously injection, every 3 days, 660 nm laser irradiation for 5 min at 6 h post-injection
-
Result:Reduced tumor volume.
Had no significant changes in mouse body weight and no obvious toxicity in major organs.
Chemical Information
-
Molecular Weight 774.02
-
Formula C47H55N3O5S
-
SMILES
O[C@]([C@@](CC[C@@]1([H])[C@@]2(CC[C@H](OC(CC/N=C3C=C4SC5=CC(N(CC)CC)=CC=C5N=C4C6=CC=CC=C/36)=O)C1)C)([H])[C@]2([H])CC7)(CC[C@@H]8C(C=C9)=COC9=O)[C@]78C
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz