Methyclothiazide
Based on 1 Customer Validation
Methyclothiazide is an orally active antihypertensive agent and a diuretic agent. Methyclothiazide leads to a reduction of the vascular response to the action of endogenous vasoconstricting stimuli, such as Norepinephrine (HY-13715). Methyclothiazide is against voltage-dependent Ca-channel (VDCC) activity in vitro.
For research use only. We do not sell to patients.
- Purity: 99.28%
- CAS No.: 135-07-9
- Formula: C9H11Cl2N3O4S2
- Molecular Weight:360.24
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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FDA-Approved Drug Library
HY-L022
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Drug Repurposing Compound Library
HY-L035
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Anti-Cardiovascular Disease Compound Library
HY-L046
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NMPA-Approved Drug Library
HY-L053
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Drug-Induced Liver Injury (DILI) Compound Library
HY-L076
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Human Metabolite Library
HY-L123
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Coagulation and Anti-coagulation Compound Library
HY-L136
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Withdrawn Drug Compound Library
HY-L140
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Off-patent Drug Library
HY-L141
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Antihypertensive Compound Library
HY-L145
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Metabolic Enzyme Compound Library
HY-L146
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Drug Repurposing Compound Library Plus
HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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FDA-Approved Drug Library Mini
HY-L022M
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Biological Activity
IC50: voltage-dependent Ca-channel (VDCC)
Methyclothiazide (0-100 μM) induces endothelium-dependent inhibition of the vasoconstrictor responses to NE and AVP only in aortas from SHR, and the maximal vasoconstrictive effect of Norepinephrine (HY-13715) and arginine vasopressin (AVP) is decreased by 59% and 32.3 %, respectively[1]. Methyclothiazide (0-100 μM) induces inhibitory effect on the contractile response to Norepinephrine (HY-13715) is abolished by N-nitro-L-arginine (NOLA) but not indomethacin[1]. Methyclothiazide (100 μM) affects the vascular responses to extracellular Ca2+ under high-K+ depolarizing conditions. It can reduce Ca2+ contracture in a high-K+, Ca2+-free solution. The maximal contracture is reduced by 90.4%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 135-07-9
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Appearance Solid
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Molecular Weight 360.24
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Formula C9H11Cl2N3O4S2
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Color White to off-white
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SMILES
O=S(C1=C(Cl)C=C(C2=C1)NC(CCl)N(C)S2(=O)=O)(N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 72 mg/mL (199.87 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Colas, B., et al., Mechanisms of methyclothiazide-induced inhibition of contractile responses in rat aorta. Eur J Pharmacol, 2000. 408(1): p. 63-7. [Content Brief]
[2]. Colas, B., et al., Direct vascular actions of methyclothiazide and indapamide in aorta of spontaneously hypertensive rats. Fundam Clin Pharmacol, 2000. 14(4): p. 363-8. [Content Brief]
[3]. Sasaki, S. and R.D. Bunag, Methyclothiazide attenuates salt-induced hypertension without affecting sympathetic responsiveness in Dahl rats. J Cardiovasc Pharmacol, 1983. 5(3): p. 378-83. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7759 mL | 13.8796 mL | 27.7593 mL | 69.3982 mL |
| 5 mM | 0.5552 mL | 2.7759 mL | 5.5519 mL | 13.8796 mL | |
| 10 mM | 0.2776 mL | 1.3880 mL | 2.7759 mL | 6.9398 mL | |
| 15 mM | 0.1851 mL | 0.9253 mL | 1.8506 mL | 4.6265 mL | |
| 20 mM | 0.1388 mL | 0.6940 mL | 1.3880 mL | 3.4699 mL | |
| 25 mM | 0.1110 mL | 0.5552 mL | 1.1104 mL | 2.7759 mL | |
| 30 mM | 0.0925 mL | 0.4627 mL | 0.9253 mL | 2.3133 mL | |
| 40 mM | 0.0694 mL | 0.3470 mL | 0.6940 mL | 1.7350 mL | |
| 50 mM | 0.0555 mL | 0.2776 mL | 0.5552 mL | 1.3880 mL | |
| 60 mM | 0.0463 mL | 0.2313 mL | 0.4627 mL | 1.1566 mL | |
| 80 mM | 0.0347 mL | 0.1735 mL | 0.3470 mL | 0.8675 mL | |
| 100 mM | 0.0278 mL | 0.1388 mL | 0.2776 mL | 0.6940 mL |
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