Methyclothiazide

Name: Methyclothiazide
Brand: MedChemExpress
SKU: HY-B0562
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0562 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

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Methyclothiazide is an orally active antihypertensive agent and a diuretic agent. Methyclothiazide leads to a reduction of the vascular response to the action of endogenous vasoconstricting stimuli, such as Norepinephrine (HY-13715). Methyclothiazide is against voltage-dependent Ca-channel (VDCC) activity in vitro.

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Methyclothiazide Chemical Structure

CAS No. : 135-07-9

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 81	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 81	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 47	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 74	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 149	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 253	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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CA CA I CA Ⅱ CA Ⅸ CA XII

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: voltage-dependent Ca-channel (VDCC)

In Vitro

Methyclothiazide (0-100 μM) induces endothelium-dependent inhibition of the vasoconstrictor responses to NE and AVP only in aortas from SHR, and the maximal vasoconstrictive effect of Norepinephrine (HY-13715) and arginine vasopressin (AVP) is decreased by 59% and 32.3 %, respectively^[1].
Methyclothiazide (0-100 μM) induces inhibitory effect on the contractile response to Norepinephrine (HY-13715) is abolished by N-nitro-L-arginine (NOLA) but not indomethacin^[1].
Methyclothiazide (100 μM) affects the vascular responses to extracellular Ca²⁺ under high-K⁺ depolarizing conditions. It can reduce Ca²⁺ contracture in a high-K⁺, Ca²⁺-free solution. The maximal contracture is reduced by 90.4%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

360.24

Formula

C₉H₁₁Cl₂N₃O₄S₂

CAS No.

135-07-9

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=C(Cl)C=C(C2=C1)NC(CCl)N(C)S2(=O)=O)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 72 mg/mL (199.87 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7759 mL	13.8796 mL	27.7593 mL
5 mM	0.5552 mL	2.7759 mL	5.5519 mL
10 mM	0.2776 mL	1.3880 mL	2.7759 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.76%

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Data Sheet (275 KB) SDS (0 KB)

COA (252 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Colas, B., et al., Mechanisms of methyclothiazide-induced inhibition of contractile responses in rat aorta. Eur J Pharmacol, 2000. 408(1): p. 63-7. [Content Brief]

[2]. Colas, B., et al., Direct vascular actions of methyclothiazide and indapamide in aorta of spontaneously hypertensive rats. Fundam Clin Pharmacol, 2000. 14(4): p. 363-8. [Content Brief]

[3]. Sasaki, S. and R.D. Bunag, Methyclothiazide attenuates salt-induced hypertension without affecting sympathetic responsiveness in Dahl rats. J Cardiovasc Pharmacol, 1983. 5(3): p. 378-83. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7759 mL	13.8796 mL	27.7593 mL	69.3982 mL
	5 mM	0.5552 mL	2.7759 mL	5.5519 mL	13.8796 mL
	10 mM	0.2776 mL	1.3880 mL	2.7759 mL	6.9398 mL
	15 mM	0.1851 mL	0.9253 mL	1.8506 mL	4.6265 mL
	20 mM	0.1388 mL	0.6940 mL	1.3880 mL	3.4699 mL
	25 mM	0.1110 mL	0.5552 mL	1.1104 mL	2.7759 mL
	30 mM	0.0925 mL	0.4627 mL	0.9253 mL	2.3133 mL
	40 mM	0.0694 mL	0.3470 mL	0.6940 mL	1.7350 mL
	50 mM	0.0555 mL	0.2776 mL	0.5552 mL	1.3880 mL
	60 mM	0.0463 mL	0.2313 mL	0.4627 mL	1.1566 mL
	80 mM	0.0347 mL	0.1735 mL	0.3470 mL	0.8675 mL
	100 mM	0.0278 mL	0.1388 mL	0.2776 mL	0.6940 mL