Mitomycin A
Based on 1 Customer Validation
Mitomycin A is an antitumor agent. Mitomycin A inhibits the spontaneous migration of mouse monocytes. Mitomycin A inhibits the production of MIF (Migration Inhibition Factor) by human lymphocytes. Mitomycin A can be used in the research of tumor diseases.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.3%
- CAS No.: 4055-39-4
- Formule: C16H19N3O6
- Masse moléculaire:349.34
-
Stockage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.006 μg/mL
Compound: 16
|
in vitro anticancer activity against cultured A549 human tumor(lung) cell line
in vitro anticancer activity against cultured A549 human tumor(lung) cell line
|
[PMID: 11585450] |
| CCRF S-180 | ED50 |
1.1 μg/mL
Compound: 28
|
Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
Antitumor activity against mouse S180 cells implanted in ip dosed ddY mouse assessed as tumor volume after 7 days
|
[PMID: 19540628] |
| CCRF S-180 | ED50 |
1.3 mg/kg
Compound: mitomycin A
|
Effective dose that gave 50 percent inhibition of sarcoma 180 tumor growth in ddY mice
Effective dose that gave 50 percent inhibition of sarcoma 180 tumor growth in ddY mice
|
[PMID: 8021919] |
| HCT-8 | IC50 |
0.006 μg/mL
Compound: 16
|
in vitro inhibition of tumor cell growth in cultured HCT-8 human tumor cell line
in vitro inhibition of tumor cell growth in cultured HCT-8 human tumor cell line
|
[PMID: 11585450] |
| HeLa | IC50 |
0.0024 μM
Compound: mitomycin A
|
In vitro anticellular activity tested against HeLa S3 cells
In vitro anticellular activity tested against HeLa S3 cells
|
[PMID: 8021918] |
| HeLa | IC50 |
0.0024 μM
Compound: mitomycin A
|
In vitro anticellular activity against HeLa S3 cells
In vitro anticellular activity against HeLa S3 cells
|
[PMID: 8021919] |
| HepG2 | IC50 |
0.008 μg/mL
Compound: 16
|
in vitro inhibition of tumor cell growth in cultured HepG2 human tumor cell line
in vitro inhibition of tumor cell growth in cultured HepG2 human tumor cell line
|
[PMID: 11585450] |
| KB | IC50 |
0.008 μg/mL
Compound: 16
|
in vitro anticancer activity against cultured KB(epidermoid) human tumor cell line
in vitro anticancer activity against cultured KB(epidermoid) human tumor cell line
|
[PMID: 11585450] |
| L1210 | IC50 |
0.0023 μM
Compound: 6
|
Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
Inhibitory concentration on multidrug-resistant L1210 leukemia cells.
|
[PMID: 1906107] |
| L1210 | IC50 |
0.0003 μM
Compound: 6
|
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
|
[PMID: 1906107] |
| L1210 | IC50 |
0.3 nM
Compound: 6
|
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
Inhibitory concentration on parentral (sensitive) L1210 leukemia cells.
|
[PMID: 1906107] |
| MCF7 | IC50 |
0.045 μg/mL
Compound: 16
|
in vitro inhibition of tumor cell growth in cultured MCF-7 human tumor cell line
in vitro inhibition of tumor cell growth in cultured MCF-7 human tumor cell line
|
[PMID: 11585450] |
| MCF7 | IC50 |
0.63 nM
Compound: 20
|
Antitumor potency on human breast MCF-7 cell lines.
Antitumor potency on human breast MCF-7 cell lines.
|
[PMID: 1906109] |
| P388 | IC50 |
0.24 nM
Compound: 20
|
Antitumor potency on P388 leukemia cells in culture
Antitumor potency on P388 leukemia cells in culture
|
[PMID: 1906109] |
| PC-3 | IC50 |
0.02 μg/mL
Compound: 16
|
in vitro inhibition of tumor cell growth in cultured PC-3 human tumor cell line
in vitro inhibition of tumor cell growth in cultured PC-3 human tumor cell line
|
[PMID: 11585450] |
| U-87MG ATCC | IC50 |
0.009 μg/mL
Compound: 16
|
in vitro anticancer activity against cultured U87MG (glioblastoma) human tumor cell line
in vitro anticancer activity against cultured U87MG (glioblastoma) human tumor cell line
|
[PMID: 11585450] |
| WiDr | IC50 |
0.052 nM
Compound: 20
|
Antitumor potency on human colon WIDR cell lines.
Antitumor potency on human colon WIDR cell lines.
|
[PMID: 1906109] |
In Vitro
Mitomycin A (0.05-5 μg/mL; 24 h) inhibits the spontaneous migration of WEHI-3 mouse monocytes in a concentration-dependent manner[1].
Mitomycin A (0.25 μg/mL; 65 h) significantly inhibits the production of migration inhibitory factor (MIF) by pokeweed mitogen-activated human lymphocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:WEHI-3 murine myelomonocytic leukemia cells
-
Concentration:0.05 μg/mL; 0.1 μg/mL; 0.5 μg/mL; 5 μg/mL
-
Incubation Time:24 h
-
Result:Exhibited a migration index (MI) of 1.01 ± 0.05 at 0.05 μg/mL.
Showed a migration index (MI) of 1.03 ± 0.03 at 0.1 μg/mL.
Reduced the mean migration index (MI) to 0.65 ± 0.01 at 0.5 μg/mL, with a statistically significant decrease.
Lowered the mean migration index (MI) to 0.52 ± 0.02 at 5 μg/mL, with a statistically significant decrease.
Caused no statistically significant decreases in migration at 0.05 and 0.1 μg/mL.
Chemical Information
-
CAS No. 4055-39-4
-
Appearance Solid
-
Masse moléculaire 349.34
-
Formule C16H19N3O6
-
Color Purple to purplish red
-
SMILES
O=C1C(C)=C(OC)C(C2=C1N3[C@@]([C@](N4)([H])[C@]4([H])C3)(OC)[C@@H]2COC(N)=O)=O
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Protocole
-
Multiplex immunofluorescence IHC
Multiplex immunofluorescence IHC detects multiple protein biomarkers in one tissue section by sequential antibody staining, HRP-mediated tyramide fluorophore deposition, heat-mediated antibody stripping, nuclear counterstaining, multispectral imaging, spectral unmixing, and digital cell phenotyping; TSA deposits fluorophore near the antigen so the fluorescence signal remains after primary and secondary antibodies are removed, enabling repeated staining cycles, including with antibodies from the same host species. Classic FFPE tumor immune-profiling applications use panels such as CD3, CD8, CD68/CD163, FOXP3, PD-1, PD-L1, pancytokeratin, Ki67, and DAPI to identify tumor cells, immune-cell subsets, checkpoint-marker expression, co-expression phenotypes, cell density, and spatial relationships in the tumor microenvironment.
-
Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
-
Multiplex immunohistochemistry
Multiplex immunohistochemistry (mIHC), also known as tyramide dignal amplification (TSA), is an enzymatic detection method that uses horseradish peroxidase (HRP) to perform high-density in-situ labeling of target proteins or nucleic acids.
Pureté et documentation
-
Fiche technique (278 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mitomycin A Related Classifications
HY-130332 Related Classifications
Powered by Bioz