2. Immunology/Inflammation Apoptosis Neuronal Signaling
  3. NOD-like Receptor (NLR) Interleukin Related Caspase Amyloid-β Tau Protein α-synuclein Pyroptosis
  4. MNP2

MNP2 is a NLRP3-ASC interaction inhibitor. MNP2 selectively binds to the PYD domain of ASC (Ka=149 nM) and blocks ASC-PYM binding (Ka=58 nM), thereby inhibiting the interaction between ASC and NLRP3 and suppressing the formation of the NLRP3 inflammasome. MNP2 inhibits IL-1β release and caspase-1 maturation, and reduces the efflux of potassium and chloride ions. MNP2 prevents mitochondrial damage and reactive oxygen species production, and significantly decreases NLRP3 inflammasome formation in neurodegenerative pathologies induced by β-amyloid, Tau protein and α-synuclein. MNP2 is applicable for the research of neurodegenerative diseases.

For research use only. We do not sell to patients.

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MNP2

MNP2 Chemical Structure

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MNP2 Related Antibodies

Top Publications Citing Use of Products

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NLRP3 NLRP1 NOD1 NOD2

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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α-synuclein α-synuclein Aggregation α-synuclein/CHMP2B

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Description

MNP2 is a NLRP3-ASC interaction inhibitor. MNP2 selectively binds to the PYD domain of ASC (Ka=149 nM) and blocks ASC-PYM binding (Ka=58 nM), thereby inhibiting the interaction between ASC and NLRP3 and suppressing the formation of the NLRP3 inflammasome. MNP2 inhibits IL-1β release and caspase-1 maturation, and reduces the efflux of potassium and chloride ions. MNP2 prevents mitochondrial damage and reactive oxygen species production, and significantly decreases NLRP3 inflammasome formation in neurodegenerative pathologies induced by β-amyloid, Tau protein and α-synuclein. MNP2 is applicable for the research of neurodegenerative diseases[1].

IC50 & Target

Caspase-1

 

IL-1β

 

NLRP3 inflammasome

 

α-synuclein

 

In Vitro

MNP2 (20-100 μM; 24 h) potently inhibits NLRP3 inflammasome activation in N9 microglia, with an IC50 of 2.57 μM for IL-1β and 16.23 μM for caspase-1, while also resisting pyroptosis induced by 1 μg/mL LPS+ATP or LPS+Aβ/Tau/α-Syn[1].
MNP2 (100 μM; 24-48 h) remains stable for 48 hours in fetal bovine serum (FBS) and for 24 hours in human serum[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MNP2 Related Antibodies

Cell Viability Assay[1]

Cell Line: N9 microglial cells
Concentration: 100 μM; stimulated with LPS (1 μg/mL), ATP (5 mM), Aβ oligomer (10 μM), Tau oligomer (5 μM), and α- Syn (5 μM)
Incubation Time: 24 h
Result: Protected cells against pyroptosis induced by LPS+ATP or LPS+Aβ/Tau/α-Syn.
Molecular Weight

1211.46

Formula

C56H94N18O12
Sequence

Val-Trp-Ile-Leu-Ala-Ala-Ile-Asn-Arg-Arg
Sequence Shortening

VWILAAINRR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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MNP2 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MNP2MNP 2MNP-2NOD-like Receptor (NLR)Interleukin RelatedCaspaseAmyloid-βTau Proteinα-synucleinPyroptosisILβ-amyloid peptideAβAbetaα-SynTauASC PYDα-SynucleinHEK 293T cellsreactive oxygen speciescaspase-1amyloid-βNLRP3 inflammasomeN9 microglial cellsinterleukin-1βInhibitorinhibitorinhibit

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