N-(2-Hydroxypropyl)methacrylamide

Name: N-(2-Hydroxypropyl)methacrylamide
Brand: MedChemExpress
SKU: HY-W077028
Rating: 4.5 (1 reviews)

Cat. No.: HY-W077028 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N-(2-Hydroxypropyl)methacrylamide is used to synthesize copolymers for the targeted delivery of antileishmanial agents in Visceral leishmaniasis (VL) .

For research use only. We do not sell to patients.

N-(2-Hydroxypropyl)methacrylamide Chemical Structure

CAS No. : 21442-01-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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5 g		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

N-(2-Hydroxypropyl)methacrylamide is used to synthesize copolymers for the targeted delivery of antileishmanial agents in Visceral leishmaniasis (VL) ^[1]^[1].

In Vivo

At 5 mg/kg body weight drug equivalent dose, all N-(2-Hydroxypropyl)methacrylamide copolymer-drug conjugates which contained lysosomally degradable side chains shows significant in vivo antileishmanial activity (>99% inhibition)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

143.18

Formula

C₇H₁₃NO₂

CAS No.

21442-01-3

Appearance

Solid

Color

White to off-white

SMILES

C=C(C)C(NCC(O)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (698.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.9842 mL	34.9211 mL	69.8422 mL
5 mM	1.3968 mL	6.9842 mL	13.9684 mL
10 mM	0.6984 mL	3.4921 mL	6.9842 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (17.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.87%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (244 KB) HNMR (125 KB) GC (125 KB)

Handling Instructions (2659 KB)

References

[1]. Nan A, et al. N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers for targeted delivery of 8-aminoquinoline antileishmanial drugs. J Control Release. 2001 Dec 13;77(3):233-43. [Content Brief]

[2]. Hao Tang, et al. Comb-like Poly(N-(2-hydroxypropyl) methacrylamide) Doxorubicin Conjugates: The Influence of Polymer Architecture and Composition on the Biological Properties. Chinese Journal of Polymer Science volume 36, pages1225-1238(2018).

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.9842 mL	34.9211 mL	69.8422 mL	174.6054 mL
	5 mM	1.3968 mL	6.9842 mL	13.9684 mL	34.9211 mL
	10 mM	0.6984 mL	3.4921 mL	6.9842 mL	17.4605 mL
	15 mM	0.4656 mL	2.3281 mL	4.6561 mL	11.6404 mL
	20 mM	0.3492 mL	1.7461 mL	3.4921 mL	8.7303 mL
	25 mM	0.2794 mL	1.3968 mL	2.7937 mL	6.9842 mL
	30 mM	0.2328 mL	1.1640 mL	2.3281 mL	5.8202 mL
	40 mM	0.1746 mL	0.8730 mL	1.7461 mL	4.3651 mL
	50 mM	0.1397 mL	0.6984 mL	1.3968 mL	3.4921 mL
	60 mM	0.1164 mL	0.5820 mL	1.1640 mL	2.9101 mL
	80 mM	0.0873 mL	0.4365 mL	0.8730 mL	2.1826 mL
	100 mM	0.0698 mL	0.3492 mL	0.6984 mL	1.7461 mL