N-(4-Anilino-1-naphthyl)maleimide
N-(4-Anilino-1-naphthyl)maleimide is a fluorescent probe that can be used to detect thiol groups in proteins. N-(4-Anilino-1-naphthyl)maleimide reacts with thiol groups and immediately produces a stable fluorescent compound with the λex=355 nm, λem=448 nm.
For research use only. We do not sell to patients.
- CAS No.: 50539-45-2
- Formula: C20H14N2O2
- Molecular Weight:314.34
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
In Vitro
N-(4-Anilino-1-naphthyl)maleimide can be used to label rabbit muscle fibers and its fluorescence spectrum reflects their conformational changes between relaxed or contracted states[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 50539-45-2
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Molecular Weight 314.34
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Formula C20H14N2O2
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SMILES
O=C(C=C1)N(C2=C3C=CC=CC3=C(NC4=CC=CC=C4)C=C2)C1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. T Honda, et al. Characterization of the reactivity of sulphydryl groups in tryptophanase by a dual-monitoring high-performance liquid chromatographic system with a site-directed fluorescent reagent. J Chromatogr. 1986 Dec 26;371:353-60. [Content Brief]
[2]. S Chaen, et al. Fluorescence properties and contraction characteristics of ANM (N-(1-anilinonaphthyl-4)maleimide)-labeled rabbit psoas muscle fibers. J Biochem. 1985 Oct;98(4):939-47. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)