N-Methylnicotinamide

Name: N-Methylnicotinamide
Brand: MedChemExpress
SKU: HY-124124
Rating: 4.5 (1 reviews)

製品番号: HY-124124 純度: 98.34%: Data Sheet SDS COA 取扱説明書
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N-Methylnicotinamide is an endogenous metabolite with antithrombotic effect. N-Methylnicotinamide via production/release of prostacyclin inhibits arterial thrombosis development. N-Methylnicotinamide is also the N-methylation product from nicotinamide catalyzed by N-methyltransferase within nicotinate and nicotinamide metabolism pathway.

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N-Methylnicotinamide 構造式

CAS 番号 : 114-33-0

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solid
500 mg	$30	在庫あり	Estimated Time of Arrival: December 31
1 g	$45	在庫あり	Estimated Time of Arrival: December 31
5 g	$95	在庫あり	Estimated Time of Arrival: December 31
10 g	$142	在庫あり	Estimated Time of Arrival: December 31
25 g	$284	在庫あり	Estimated Time of Arrival: December 31
50 g	$425	在庫あり	Estimated Time of Arrival: December 31
> 100 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Other Forms of N-Methylnicotinamide:

N-Methylnicotinamide-d₄ 在庫あり
N-Methylnicotinamide (Standard) 在庫あり

顧客検証

•Biochem Pharmacol. 2024 Dec;230(Pt 2):116593. [Abstract]

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
Oocyte	IC₅₀	13661 μM Compound: N1-methylnicotinamide	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes	[PMID: 12176030]
Oocyte	IC₅₀	1560 μM Compound: N1-methylnicotinamide	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 100 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of TEA uptake (TEA: 100 uM) in Xenopus laevis oocytes	[PMID: 10082798]
Oocyte	IC₅₀	7337 μM Compound: N1-methylnicotinamide	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 1 uM) in Xenopus laevis oocytes	[PMID: 12176030]

体内実験

N-Methylnicotinamide (3-30 mg/kg, i.v., 10 min before the induction of arterial thrombosis) decreases the arterial thrombus weight, collagen induced platelet aggregation and decreases PAI-1 antigen and activity in plasma without changing t-PA activity in 2K-1C hypertensive rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	2K-1C hypertensive rats^[1]
Dosage:	3-30 mg/kg
Administration:	i.v., 10 min before the induction of arterial thrombosis
Result:	Resulted in a dose-dependent decrease of arterial thrombus weight, decreased PAI-1 antigen and activity in plasma in dose-dependent manner, whereas it did not change t-PA activity, decreased collagen induced platelet aggregation in dose-dependent manner.

Animal Model:	Weanling male rats^[3]
Dosage:	30-300 mg/kg
Administration:	mixed in the diet, daily for 31 days
Result:	Significantly increased the liver weight, statistically significantly the content of NAD and total nicotinamide in liver and the content of NAD in blood in rats.

分子量

136.15

分子式

C₇H₈N₂O

CAS 番号

114-33-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CN=C1)NC

Structure Classification

Initial Source

Endogenous metabolite

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 200 mg/mL (1468.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.3448 mL	36.7242 mL	73.4484 mL
5 mM	1.4690 mL	7.3448 mL	14.6897 mL
10 mM	0.7345 mL	3.6724 mL	7.3448 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (15.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.17 mg/mL (15.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.17 mg/mL (15.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.63%

Select Batch:

データシート (275 KB) SDS (393 KB)

COA (248 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Mogielnicki A, et al. N-methylnicotinamide inhibits arterial thrombosis in hypertensive rats. J Physiol Pharmacol. 2007 Sep;58(3):515-27. [Content Brief]

[2]. Bi HC, et al. N-methylnicotinamide and nicotinamide N-methyltransferase are associated with microRNA-1291-altered pancreatic carcinoma cell metabolome and suppressed tumorigenesis. Carcinogenesis. 2014 Oct;35(10):2264-72. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	7.3448 mL	36.7242 mL	73.4484 mL	183.6210 mL
	5 mM	1.4690 mL	7.3448 mL	14.6897 mL	36.7242 mL
	10 mM	0.7345 mL	3.6724 mL	7.3448 mL	18.3621 mL
	15 mM	0.4897 mL	2.4483 mL	4.8966 mL	12.2414 mL
	20 mM	0.3672 mL	1.8362 mL	3.6724 mL	9.1811 mL
	25 mM	0.2938 mL	1.4690 mL	2.9379 mL	7.3448 mL
	30 mM	0.2448 mL	1.2241 mL	2.4483 mL	6.1207 mL
	40 mM	0.1836 mL	0.9181 mL	1.8362 mL	4.5905 mL
	50 mM	0.1469 mL	0.7345 mL	1.4690 mL	3.6724 mL
	60 mM	0.1224 mL	0.6121 mL	1.2241 mL	3.0604 mL
	80 mM	0.0918 mL	0.4591 mL	0.9181 mL	2.2953 mL
	100 mM	0.0734 mL	0.3672 mL	0.7345 mL	1.8362 mL