Nicotinamide riboside chloride

Name: Nicotinamide riboside chloride
Brand: MedChemExpress
SKU: HY-123033A
Rating: 5.0 (9 reviews)

Cat. No.: HY-123033A Purity: 99.59%: FAQs
Data Sheet SDS COA Handling Instructions

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Nicotinamide riboside Chloride, an orally active NAD⁺ precursor, increases NAD⁺ levels and activates SIRT1 and SIRT3. Nicotinamide riboside Chloride is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities. Nicotinamide riboside Chloride reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease.

For research use only. We do not sell to patients.

Nicotinamide riboside chloride Chemical Structure

CAS No. : 23111-00-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 9 publication(s) in Google Scholar

Other Forms of Nicotinamide riboside chloride:

Nicotinamide riboside Get quote
Nicotinamide riboside tartrate Get quote
Nicotinamide riboside malate Get quote

9 Publications Citing Use of MCE Nicotinamide riboside chloride

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Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

Biological Activity
Purity & Documentation
References
Customer Review

Description

Nicotinamide riboside Chloride, an orally active NAD⁺ precursor, increases NAD⁺ levels and activates SIRT1 and SIRT3. Nicotinamide riboside Chloride is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities^[1]. Nicotinamide riboside Chloride reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease^[2].

IC₅₀ & Target^[1]

SIRT1

SIRT3

Human Endogenous Metabolite

In Vitro

Nicotinamide riboside Chloride (0.5 mM; 24 hours) reduces the acetylation status of Ndufa9 and SOD2^[1].
Nicotinamide riboside Chloride increases intracellular and mitochondrial NAD⁺ content in C2C12, Hepa1.6, and HEK293 cells in a concentration-dependent manner at concentrations ranging from 1-1000 μM^[1].
Nicotinamide riboside chloride boosts NAD to restore antiviral poly(ADP-ribose) polymerase (PARP) functions to support innate immunity for coronavirus (CoVs), a cause of COVID-19^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK293T cells
Concentration:	0.5 mM
Incubation Time:	24 hours
Result:	Reduced the acetylation status of Ndufa9 and SOD2.

In Vivo

Chronic Nicotinamide riboside Chloride (p.o.; 400 mg/kg/day; for 16 weeks) supplementation increases plasma and intracellular NAD⁺ content in a tissue-specific manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10-week-old C57Bl/6J mice^[1]
Dosage:	400 mg/kg
Administration:	PO; daily; for 16 weeks
Result:	Increased plasma and intracellular NAD⁺ content in a tissue-specific manner.

Clinical Trial

Molecular Weight

290.70

Formula

C₁₁H₁₅ClN₂O₅

CAS No.

23111-00-4

Appearance

Solid

Color

White to yellow

SMILES

NC(C1=C[N+]([C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)=CC=C1)=O.[Cl-]

Structure Classification

Initial Source

Animals

milk and beer

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (430.00 mM; Need ultrasonic)

DMSO : 50 mg/mL (172.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4400 mL	17.1999 mL	34.3997 mL
5 mM	0.6880 mL	3.4400 mL	6.8799 mL
10 mM	0.3440 mL	1.7200 mL	3.4400 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 25 mg/mL (86.00 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (249 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Cantó C, et al. The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protectsagainst high-fat diet-induced obesity. Cell Metab. 2012 Jun 6;15(6):838-47. [Content Brief]

[2]. Bing Gong, et al. Nicotinamide Riboside Restores Cognition Through an Upregulation of Proliferator-Activated Receptor-γ Coactivator 1α Regulated β-Secretase 1 Degradation and Mitochondrial Gene Expression in Alzheimer's Mouse Models. Neurobiol Aging. 2013 Jun;34(6):1581-8. [Content Brief]

[3]. Collin D Heer, et al. Coronavirus and PARP Expression Dysregulate the NAD Metabolome: A Potentially Actionable Component of Innate Immunity. bioRxiv. 2020 Apr 30;2020.04.17.047480. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.4400 mL	17.1999 mL	34.3997 mL	85.9993 mL
	5 mM	0.6880 mL	3.4400 mL	6.8799 mL	17.1999 mL
	10 mM	0.3440 mL	1.7200 mL	3.4400 mL	8.5999 mL
	15 mM	0.2293 mL	1.1467 mL	2.2933 mL	5.7333 mL
	20 mM	0.1720 mL	0.8600 mL	1.7200 mL	4.3000 mL
	25 mM	0.1376 mL	0.6880 mL	1.3760 mL	3.4400 mL
	30 mM	0.1147 mL	0.5733 mL	1.1467 mL	2.8666 mL
	40 mM	0.0860 mL	0.4300 mL	0.8600 mL	2.1500 mL
	50 mM	0.0688 mL	0.3440 mL	0.6880 mL	1.7200 mL
	60 mM	0.0573 mL	0.2867 mL	0.5733 mL	1.4333 mL
	80 mM	0.0430 mL	0.2150 mL	0.4300 mL	1.0750 mL
	100 mM	0.0344 mL	0.1720 mL	0.3440 mL	0.8600 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.