Nicotinamide riboside malate

Name: Nicotinamide riboside malate
Brand: MedChemExpress
SKU: HY-123033C
Rating: 5.0 (25 reviews)

Cat. No.: HY-123033C Purity: 98.04%: Data Sheet
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Nicotinamide riboside malate, an orally active NAD⁺ precursor, increases NAD⁺ levels and activates SIRT1 and SIRT3. Nicotinamide riboside malate is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities. Nicotinamide riboside malate reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease.

For research use only. We do not sell to patients.

CAS No. : 2415659-01-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 25 publication(s) in Google Scholar

Other Forms of Nicotinamide riboside malate:

Nicotinamide riboside chloride In-stock
Nicotinamide riboside tartrate In-stock
Nicotinamide riboside Get quote
Nicotinamide riboside triflate Get quote

25 Publications Citing Use of MCE Nicotinamide riboside malate

Bio/Physico-chemical Assay

Histological Imaging/Staining

Cell Imaging/Staining

In Vivo Efficacy Study

IHC

2D/3D Cell Culture and Differentiation

Cell Proliferation/Viability Assay

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 26;13(1):18. [Abstract]; Effect of SIRT3 agonist (Nicotinamide riboside chloride; 0-1600 μM) on the proliferation of MC3T3-E1 cells was determined by CCK-8 assay after 2 days of stimulation

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 26;13(1):18. [Abstract]; IF staining of SIRT3, BNIP3, NIX and DRP1 expression in MC3T3-E1 cells treated with DEX and SIRT3 agonist (800 μm).

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Bone Res. 2025 Jan 26;13(1):18. [Abstract]; WB analysis of SIRT3, BNIP3, NIX, DRP1, MFN1 and MFN2 expression in MC3T3-E1 cells treated with DEX and SIRT3 agonist (800 μM).

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Res Sq. 2025 Mar 31.; Representative hematoxylin and eosin (H&E)-stained sections of small intestines were collected at the end of the experiment (NR:Nicotinamide riboside,400 mg/kg/day； po for 4 weeks) or vehicle (phosphate buffer; at the same dose and volume as NR) once a day after irradiation exposure in C57BL/6 mouse)

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Res Sq. 2025 Mar 31.; Immunofluorescence detection of Ki-67 and ZO-1 in IECs between groups with NR (Nicotinamide riboside; 0.5 mM; 24 h).

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Res Sq. 2025 Mar 31.; Morphology of the SI and colon (NR:Nicotinamide riboside,400 mg/kg/day； po for 4 weeks) or vehicle (phosphate buffer; at the same dose and volume as NR) once a day after irradiation exposure in C57BL/6 mouse)

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2024 Jul 1;65(8):1. [Abstract]; The effect of different concentrations of NR (Nicotinamide riboside chloride;200,400, 800, 1000 μM) on primary human trabecular meshwork cells' viability after 24 and 72 hours of treatment, was determined by using the CCK-8 assay

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Aug 9:117066. [Abstract]; WB images of SIRT3, AcK68-MnSOD and AcK122-MnSOD expressions in DRG tissues. Quantification of SIRT3, AcK68-MnSOD and AcK122-MnSOD expressions normalized with β-actin expressions and the Saline+Saline treated samples was set to 1 (NR (Nicotinamide riboside chloride); PTX (4 mg/kg, i. p.) Group, PTX (4 mg/kg, i.p.) + NR (400 mg/kg, i.g.) Group, PTX (4 mg/kg, I.P.) + NR (400 mg/kg, i.g.) +3-TYP (50 mg/kg, i.p.) group, NR (400 mg/kg, i.p.) group, 3-TYP (50 mg/kg, i.p.) group in SIRT3 WT mice)

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Aug 9:117066. [Abstract]; The representative images depicting the expression of AcK68-MnSOD in each group were utilized for immunohistochemical detection (NR (Nicotinamide riboside chloride); PTX (4 mg/kg, i. p.) Group, PTX (4 mg/kg, i.p.) + NR (400 mg/kg, i.g.) Group, PTX (4 mg/kg, I.P.) + NR (400 mg/kg, i.g.) +3-TYP (50 mg/kg, i.p.) group, NR(400 mg/kg, i.p.) group, 3-TYP (50 mg/kg, i.p.) group in SIRT3 WT mice)

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Cells. 2023 Oct 2;12(19):2396. [Abstract]; THP-1 cells were supplemented with 200 µM NR (Nicotinamide riboside chloride) for 4 h before treatment with CEES or HN2 in growth medium (“Ctrl”) for 30 min. The “0” refers to solvent control. After exposure, cells were incubated with fresh growth medium with or without 200 µM NR for 20 h, and cell viability was measured with alamarBlue assay

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2023 Mar 2;6(1):235. [Abstract]; The cells were pretreated with NR (0.5 µM) for 1 h followed by post-incubation with LPS (1 µg/mL) or H2O2 (100 µM) for 24 h, quantitative analysis of cell viability in each group after LPS stimulation and H2O2 stimulation.

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Nov:57:102507. [Abstract]; MitoParaquat (0.1 μM, 30 min) abolished the inhibition of platelet aggregation by nicotinamide riboside (1 mM, 30 min) or olaparib (20 μM, 30 min) in resting platelets from LST patients.

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Nov:57:102507. [Abstract]; MitoParaquat (0.1 μM, 30 min) did not interrupt the improvements in mitochondrial respiration induced by nicotinamide riboside (1 mM) or olaparib (20 μM) in resting platelets from LST patients.

: Nicotinamide riboside malate purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2021 Nov 10;11(1):192.; Relative ATP, NAD+, NADH, and NAD+/NADH levels in NR (Nicotinamide ribosid, 100 mM, 24 h) treatment SY5Y cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Nicotinamide riboside malate, an orally active NAD⁺ precursor, increases NAD⁺ levels and activates SIRT1 and SIRT3. Nicotinamide riboside malate is a source of vitamin B3 (niacin) and enhances oxidative metabolism, protection against high fat diet-induced metabolic abnormalities^[1]. Nicotinamide riboside malate reduces cognitive deterioration in a transgenic mouse model of Alzheimer’s disease^[2].

IC₅₀ & Target^[1]

SIRT1

SIRT3

Human Endogenous Metabolite

In Vitro

Nicotinamide riboside malate (0.5 mM; 24 hours) reduces the acetylation status of Ndufa9 and SOD2^[1].
Nicotinamide riboside malate increases intracellular and mitochondrial NAD⁺ content in C2C12, Hepa1.6, and HEK293 cells in a concentration-dependent manner at concentrations ranging from 1-1000 μM^[1].
Nicotinamide riboside malate boosts NAD to restore antiviral poly(ADP-ribose) polymerase (PARP) functions to support innate immunity for coronavirus (CoVs), a cause of COVID-19^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HEK293T cells
Concentration:	0.5 mM
Incubation Time:	24 hours
Result:	Reduced the acetylation status of Ndufa9 and SOD2.

In Vivo

Chronic Nicotinamide riboside malate (p.o.; 400 mg/kg/day; for 16 weeks) supplementation increases plasma and intracellular NAD⁺ content in a tissue-specific manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	10-week-old C57Bl/6J mice^[1]
Dosage:	400 mg/kg
Administration:	PO; daily; for 16 weeks
Result:	Increased plasma and intracellular NAD⁺ content in a tissue-specific manner.

Molecular Weight

388.33

Formula

C₁₅H₂₀N₂O₁₀

CAS No.

2415659-01-5

Appearance

Solid

Color

White to off-white

SMILES

NC(C1=C[N+]([C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)=CC=C1)=O.O[C@H](C(O)=O)CC([O-])=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 116.67 mg/mL (300.44 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5751 mL	12.8756 mL	25.7513 mL
5 mM	0.5150 mL	2.5751 mL	5.1503 mL
10 mM	0.2575 mL	1.2876 mL	2.5751 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.04%

Select Batch:

Data Sheet (281 KB) SDS (251 KB)

COA (255 KB) HNMR (290 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Cantó C, et al. The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protectsagainst high-fat diet-induced obesity. Cell Metab. 2012 Jun 6;15(6):838-47. [Content Brief]

[2]. Bing Gong, et al. Nicotinamide Riboside Restores Cognition Through an Upregulation of Proliferator-Activated Receptor-γ Coactivator 1α Regulated β-Secretase 1 Degradation and Mitochondrial Gene Expression in Alzheimer's Mouse Models. Neurobiol Aging. 2013 Jun;34(6):1581-8. [Content Brief]

[3]. Collin D Heer, et al. Coronavirus and PARP Expression Dysregulate the NAD Metabolome: A Potentially Actionable Component of Innate Immunity. bioRxiv. 2020 Apr 30;2020.04.17.047480. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.5751 mL	12.8756 mL	25.7513 mL	64.3782 mL
	5 mM	0.5150 mL	2.5751 mL	5.1503 mL	12.8756 mL
	10 mM	0.2575 mL	1.2876 mL	2.5751 mL	6.4378 mL
	15 mM	0.1717 mL	0.8584 mL	1.7168 mL	4.2919 mL
	20 mM	0.1288 mL	0.6438 mL	1.2876 mL	3.2189 mL
	25 mM	0.1030 mL	0.5150 mL	1.0301 mL	2.5751 mL
	30 mM	0.0858 mL	0.4292 mL	0.8584 mL	2.1459 mL
	40 mM	0.0644 mL	0.3219 mL	0.6438 mL	1.6095 mL
	50 mM	0.0515 mL	0.2575 mL	0.5150 mL	1.2876 mL
	60 mM	0.0429 mL	0.2146 mL	0.4292 mL	1.0730 mL
	80 mM	0.0322 mL	0.1609 mL	0.3219 mL	0.8047 mL
	100 mM	0.0258 mL	0.1288 mL	0.2575 mL	0.6438 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.