Cat. No.: HY-N2410

N-trans-Feruloyltyramine

(Synonyms: N-feruloyltyramine; Moupinamide)

Customer Review

Based on 1 Customer Validation

N-trans-Feruloyltyramine (N-feruloyltyramine), an alkaloid, is a potent antioxidant. N-trans-Feruloyltyramine improves H₂O₂-induced intracellular ROS generation and decreases apoptosis. N-trans-Feruloyltyramine has the potential for oxidative stress-related neurodegenerative conditions and cancer research.

For research use only. We do not sell to patients.

Purity: 99.90%
CAS No.: 66648-43-9
Formula: C₁₈H₁₉NO₄
Molecular Weight:313.35

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

N-trans-Feruloyltyramine Chemical Structure

CAS No.: 66648-43-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	13.35 μg/mL Compound: 6	Cytotoxicity against human A549 cells after 7 days Cytotoxicity against human A549 cells after 7 days	[PMID: 1593281]
A549	IC₅₀	>30 μM Compound: 7	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 22951040]
A549	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human A549 cells after 3 days by SRB assay Antiproliferative activity against human A549 cells after 3 days by SRB assay	[PMID: 25241925]
BV-2	IC₅₀	17.36 μM Compound: 7	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction	[PMID: 22951040]
BV-2	IC₅₀	18 μM Compound: 21	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay	[PMID: 33822610]
DU-145	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human DU145 cells after 3 days by SRB assay Antiproliferative activity against human DU145 cells after 3 days by SRB assay	[PMID: 25241925]
Erythrocyte	IC₅₀	0.88 mM Compound: 25	Anticomplement activity in sheep erythrocytes assessed as concentration required for 50% hemolytic inhibition by classic pathway pretreated for 10 mins with guinea pig serum followed by erythrocyte addition measured after 30 mins by spectrophotometeric me Anticomplement activity in sheep erythrocytes assessed as concentration required for 50% hemolytic inhibition by classic pathway pretreated for 10 mins with guinea pig serum followed by erythrocyte addition measured after 30 mins by spectrophotometeric me	[PMID: 29631958]
Erythrocyte	IC₅₀	1.33 mM Compound: 25	Anticomplement activity in rabbit erythrocytes assessed as concentration required for 50% hemolytic inhibition by alternative pathway pretreated for 10 mins with normal human serum followed by erythrocyte addition measured after 30 mins by spectrophotomet Anticomplement activity in rabbit erythrocytes assessed as concentration required for 50% hemolytic inhibition by alternative pathway pretreated for 10 mins with normal human serum followed by erythrocyte addition measured after 30 mins by spectrophotomet	[PMID: 29631958]
GES1	IC₅₀	>150 μM Compound: 14	Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HCT-15	IC₅₀	>30 μM Compound: 7	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 22951040]
HepG2	IC₅₀	6.6 μg/mL Compound: 6	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 11374955]
HepG2 2.2.15	IC₅₀	1.9 μg/mL Compound: 6	Cytotoxicity against human HepG2(2.2.15) cells by MTT assay Cytotoxicity against human HepG2(2.2.15) cells by MTT assay	[PMID: 11374955]
HGC-27	IC₅₀	84.5 μM Compound: 14	Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HT-29	ED₅₀	23.58 μg/mL Compound: 6	Cytotoxicity against human HT-29 cells after 7 days Cytotoxicity against human HT-29 cells after 7 days	[PMID: 1593281]
HT-29	IC₅₀	>20 μM Compound: 17	Cytotoxicity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs Cytotoxicity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs	[PMID: 26147490]
KB	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human KB cells after 3 days by SRB assay Antiproliferative activity against human KB cells after 3 days by SRB assay	[PMID: 25241925]
KB	IC₅₀	>47.8 μM Compound: 12	Cytotoxicity against human KB cells by sulforhodamine B assay Cytotoxicity against human KB cells by sulforhodamine B assay	[PMID: 26928423]
LNCaP	IC₅₀	>100 μM Compound: 5	Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 11858758]
MCF7	ED₅₀	4.76 μg/mL Compound: 6	Cytotoxicity against human MCF7 cells after 7 days Cytotoxicity against human MCF7 cells after 7 days	[PMID: 1593281]
MCF7	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 25241925]
MDA-MB-231	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay Antiproliferative activity against human MDA-MB-231 cells after 3 days by SRB assay	[PMID: 25241925]
MDA-MB-435	IC₅₀	>20 μM Compound: 17	Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell growth incubated for 72 hrs Cytotoxicity against human MDA-MB-435 cells assessed as reduction in cell growth incubated for 72 hrs	[PMID: 26147490]
MGC-803	IC₅₀	98.3 μM Compound: 14	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
RAW264.7	IC₅₀	>50 μM Compound: 25	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 33939429]
RAW264.7	IC₅₀	6.33 μM Compound: 9	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs	[PMID: 31126853]
SK-BR-3	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human SK-BR-3 cells after 3 days by SRB assay Antiproliferative activity against human SK-BR-3 cells after 3 days by SRB assay	[PMID: 25241925]
SK-MEL-2	IC₅₀	>30 μM Compound: 7	Cytotoxicity against human SK-MEL-2 cells by SRB assay Cytotoxicity against human SK-MEL-2 cells by SRB assay	[PMID: 22951040]
SK-OV-3	IC₅₀	>30 μM Compound: 7	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 22951040]
Vero	IC₅₀	>47.8 μM Compound: 12	Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay Cytotoxicity against African green monkey Vero cells by sulforhodamine B assay	[PMID: 26928423]
ZR-75-1	IC₅₀	>40 μM Compound: 24	Antiproliferative activity against human ZR-75-1 cells after 3 days by SRB assay Antiproliferative activity against human ZR-75-1 cells after 3 days by SRB assay	[PMID: 25241925]

In Vitro

N-trans-Feruloyltyramine (N-feruloyltyramine; 10-500 µM; 3 hours prior H₂O₂) protects the cells against H₂O₂-induced toxicity^[1].
N-trans-Feruloyltyramine (25-100 µM; 3 hours prior H₂O₂) with 100 µM significantly reduces Bax and activated caspase-3 levels in H₂O₂-treated SK-N-SH cells^[1].
N-trans-Feruloyltyramine significantly improves an H₂O₂-mediated increase in ROS levels^[1].
N-trans-Feruloyltyramine (10-500 µM) does not affect viability of the SK-N-SH cells^[1].
N-trans-Feruloyltyramine (64-320 µM; 24 hours) shows proliferation inhibition on HepG2 cells with IC₅₀ value of 194 µM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

SK-N-SH cells
Concentration:

10, 25, 50, 100, 150, 250, 500 µM
Incubation Time:

For 3 hours prior H₂O₂
Result:

Protected the cells against H₂O₂ (150 µM)-induced toxicity as determined by a significant higher percentage of viability in cells.

Western Blot Analysis^[1]

Cell Line:

SK-N-SH cells
Concentration:

25, 50, 100 μM
Incubation Time:

For 3 hours prior H₂O₂
Result:

Abolished H₂O₂-induced Bax expression.
Significantly decreased activated caspase-3 levels.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 66648-43-9
Appearance Solid
Molecular Weight 313.35
Formula C₁₈H₁₉NO₄
Color White to off-white
SMILES

O=C(NCCC1=CC=C(O)C=C1)/C=C/C2=CC=C(O)C(OC)=C2
Synonyms

N-feruloyltyramine; Moupinamide
Structure Classification
- Alkaloids
- Other Alkaloids
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (319.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1913 mL

15.9566 mL

31.9132 mL

5 mM

0.6383 mL

3.1913 mL

6.3826 mL

10 mM

0.3191 mL

1.5957 mL

3.1913 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (398 KB)
- English - EN (398 KB)
- Français - FR (398 KB)
- Deutsch - DE (398 KB)
- Norwegian - NO (398 KB)
- Español - ES (398 KB)
- Swedish - SV (398 KB)
- Italian - IT (398 KB)
- Korean - KR (398 KB)
- Portuguese - PT (398 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Rungtip Soi-ampornkul, et al. N-trans-feruloyltyramine Protects Human Neuroblastoma SK-N-SH Cell Line Against H2O2-Induced Cytotoxicity. Natural Product CommunicationsVolume 17, Issue 8, August 2022.

[2]. Xudong Gao, et al. Effects of N-trans-feruloyltyramine isolated from laba garlic on antioxidant, cytotoxic activities and H2O2-induced oxidative damage in HepG2 and L02 cells. Food Chem Toxicol. 2019 Aug:130:130-141. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1913 mL	15.9566 mL	31.9132 mL	79.7830 mL
	5 mM	0.6383 mL	3.1913 mL	6.3826 mL	15.9566 mL
	10 mM	0.3191 mL	1.5957 mL	3.1913 mL	7.9783 mL
	15 mM	0.2128 mL	1.0638 mL	2.1275 mL	5.3189 mL
	20 mM	0.1596 mL	0.7978 mL	1.5957 mL	3.9891 mL
	25 mM	0.1277 mL	0.6383 mL	1.2765 mL	3.1913 mL
	30 mM	0.1064 mL	0.5319 mL	1.0638 mL	2.6594 mL
	40 mM	0.0798 mL	0.3989 mL	0.7978 mL	1.9946 mL
	50 mM	0.0638 mL	0.3191 mL	0.6383 mL	1.5957 mL
	60 mM	0.0532 mL	0.2659 mL	0.5319 mL	1.3297 mL
	80 mM	0.0399 mL	0.1995 mL	0.3989 mL	0.9973 mL
	100 mM	0.0319 mL	0.1596 mL	0.3191 mL	0.7978 mL

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N-trans-Feruloyltyramine

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References

Help & FAQs

Complete Stock Solution Preparation Table

N-trans-Feruloyltyramine Related Classifications

HY-N2410 Related Classifications