N3-D-Dap(Fmoc)-OH
Based on 1 Customer Validation
N3-D-Dap(Fmoc)-OH is a click chemistry reagent. N3-D-Dap(Fmoc)-OH can be used as a component for coupling by click reaction and as an orthogonally protected diaminocarboxylic acid derivative. N3-D-Dap(Fmoc)-OH contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. N3-D-Dap(Fmoc)-OH can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity : 99.96%
- CAS No.: 1807631-13-5
- Formula: C18H16N4O4
- Molecular Weight:352.34
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
N3-D-Dap (Fmoc)-OH (4 equiv.; 1 h) is a building block in solid-phase synthesis, which can be used to prepare azidopeptide 13 derived from iturin A for the generation of azidopeptides amenable to subsequent click chemistry modification[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1807631-13-5
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Appearance Solid
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Molecular Weight 352.34
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Formula C18H16N4O4
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Color White to off-white
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SMILES
[N-]=[N+]=N[C@@H](C(O)=O)CNC(OCC1C2=CC=CC=C2C3=CC=CC=C31)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (283.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8382 mL | 14.1908 mL | 28.3817 mL | 70.9542 mL |
| 5 mM | 0.5676 mL | 2.8382 mL | 5.6763 mL | 14.1908 mL | |
| 10 mM | 0.2838 mL | 1.4191 mL | 2.8382 mL | 7.0954 mL | |
| 15 mM | 0.1892 mL | 0.9461 mL | 1.8921 mL | 4.7303 mL | |
| 20 mM | 0.1419 mL | 0.7095 mL | 1.4191 mL | 3.5477 mL | |
| 25 mM | 0.1135 mL | 0.5676 mL | 1.1353 mL | 2.8382 mL | |
| 30 mM | 0.0946 mL | 0.4730 mL | 0.9461 mL | 2.3651 mL | |
| 40 mM | 0.0710 mL | 0.3548 mL | 0.7095 mL | 1.7739 mL | |
| 50 mM | 0.0568 mL | 0.2838 mL | 0.5676 mL | 1.4191 mL | |
| 60 mM | 0.0473 mL | 0.2365 mL | 0.4730 mL | 1.1826 mL | |
| 80 mM | 0.0355 mL | 0.1774 mL | 0.3548 mL | 0.8869 mL | |
| 100 mM | 0.0284 mL | 0.1419 mL | 0.2838 mL | 0.7095 mL |
N3-D-Dap(Fmoc)-OH Related Classifications
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