Nile Blue A perchlorate
Nile Blue A perchlorate is a cationic oxazine dye and a laser dye for pulsed and continuous-wave operations (Ex/Em = 365/660 nm). Nile Blue A perchlorate exhibits strong solvent coloration properties and is highly sensitive to environmental polarity and pH values.
For research use only. We do not sell to patients.
- CAS No.: 53340-16-2
- Formula: C20H20ClN3O5
- Molecular Weight:417.84
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended solution. This solution merely provides guidance; it should be modified according to your specific needs.)
Preparation: Prepare a 1% dye aqueous solution. Heat gently to ensure complete dissolution of the dye, and filter the solution before use.
Staining: The slides of cells or tissues are heat-fixed, and then they are stained in the staining solution at 55°C for 10 minutes.
Differentiation: Rinse the slides with water to remove excess dye, and then rinse them for 1 minute with an 8% acetic acid aqueous solution for differentiation.
Observation: After rinsing with water again and drying, use a transmitted fluorescence microscope (excitation wavelength ≈ 400 nm) for observation. Bright orange fluorescent particles can be seen.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 53340-16-2
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Molecular Weight 417.84
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Formula C20H20ClN3O5
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SMILES
O=Cl(=O)([O-])=O.NC1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)N=C2C4=C1C=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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