OM-7D3-B3

Cat. No.: HY-P991200 Purity: 97.98%: Data Sheet
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OM-7D3-B3 is an antibody-based antiviral agent targeting the tight junction protein CLDN1 (K_d=4 nM). By binding to the first extracellular domain of CLDN1, OM-7D3-B3 disrupts the formation of the CLDN1-CD81 co-receptor complex, thereby effectively inhibiting the entry of hepatitis C virus (HCV). OM-7D3-B3 not only prevents de novo and chronic HCV infections in humanized liver chimeric mice and uPA-SCID mice transplanted with human livers, but also exhibits favorable safety with no toxic effects observed. OM-7D3-B3 serves as a critical tool for research on HCV infection mechanisms and antiviral drug development.

For research use only. We do not sell to patients.

OM-7D3-B3 Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

Claudin-1

4 nM (Kd)

In Vitro

OM-7D3-B3 (20 μg/mL) specifically binds to CLDN1 on the surface of Huh7.5.1 cells, HepG2 cells, primary human hepatocytes, and 293T cells overexpressing CLDN1^[1].
OM-7D3-B3 partially inhibits the entry of HCV pseudovirions (genotypes 1a and 1b) into primary human hepatocytes^[1].
Serial dilutions of OM-7D3-B3 dose-dependently inhibit infection of Huh7.5.1 cells by wild-type and DAA-resistant HCVcc, with IC₅₀ values of 0.13 μg/mL, 0.23 μg/mL, and 0.36 μg/mL against Jc1, Jc1-A156S, and Jc1-R155K, respectively^[1].
OM-7D3-B3 (11 μg/mL; 24 h) effectively inhibits cell-to-cell transmission of HCV between Huh7.5.1 producer cells and target cells^[1].
When used in combination with Sofosbuvir (HY-15005) and Daclatasvir (HY-10466), OM-7D3-B3 exhibits synergistic antiviral activity against HCVcc infection in Huh7.5.1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Huh7.5.1 producer and target cells
Concentration:	11 μg/mL
Incubation Time:	24 h
Result:	Inhibited HCV cell-cell transmission between Huh7.5.1 producer and target cells

In Vivo

The anti-CLDN1 monoclonal antibody OM-7D3-B3 (25 mg/kg; i.p.; weekly; 4 weeks) reverses chronic HCV infection in human-liver chimeric uPA-SCID mice^[1].
OM-7D3-B3 prevents HCV infection in human liver-grafted uPA-SCID mice by binding the first extracellular domain of CLDN-1^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	uPA-SCID (severe combined immunodeficient, homologous for urokinase-type plasminogen activator expression under control of the mouse albumin promoter, human-liver chimeric, engrafted with primary human hepatocytes, HCV-infected)^[1]
Dosage:	25 mg/kg
Administration:	i.p.; weekly; 4 weeks
Result:	Cleared chronic HCV infection, resulting in undetectable HCV RNA levels at the end of the study period. Maintained stable human albumin levels, confirming preserved human hepatocyte engraftment and no adverse effects on liver function.

Gene ID

9076 [NCBI]

Accession

O95832

Application

ELISA, FACS, Functional assay

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Appearance

Liquid

Color

Colorless to light yellow

SMILES

N/A

Shipping

Shipping with dry ice.

Formulation

Please refer to the lot-specific COA for specific buffer information.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Purity: 97.98%

Select Batch:

Data Sheet (261 KB) SDS (251 KB)

COA (231 KB) SDS-PAGE (68 KB) SEC-HPLC (80 KB)

Inhibitory Antibodies User Guide (603 KB)

References

[1]. Colpitts CC, et al. Humanisation of a claudin-1-specific monoclonal antibody for clinical prevention and cure of HCV infection without escape. Gut. 2018;67(4):736-745. [Content Brief]

[2]. Hashimoto Y, et al. Claudin-targeted drug development using anti-claudin monoclonal antibodies to treat hepatitis and cancer. Ann N Y Acad Sci. 2017;1397(1):5-16. [Content Brief]

OM-7D3-B3 Related Classifications

Infection

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OM-7D3-B3HCVClaudinHepatitis C virus293T cellsHuh7.5.1human-liver chimeric miceclaudin-1CLDN1HepG2CD81uPA-SCID miceprimary human hepatocytesInhibitorinhibitorinhibit

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