Ophiobolin B
Based on 1 Customer Validation
Ophiobolin B, a sesterterpene metabolite of Helminthosporium oryzae, inhibits proton extrusion from maize coleoptiles. Ophiobolin B inhibits fusicoccin (FC) promoted proton extrusion, potassium uptake and cell enlargement. The MIC values with the antifungal effect of Ophiobolins B on different zygomycetes is 25–50 μg/mL.
For research use only. We do not sell to patients.
- Purity : 99.53%
- CAS No.: 5601-74-1
- Formula: C25H38O4
- Molecular Weight:402.57
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
Fungal[2]
Chemical Information
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CAS No. 5601-74-1
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Appearance Solid
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Molecular Weight 402.57
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Formula C25H38O4
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Color White to off-white
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SMILES
C[C@@]12C[C@@]([C@@]3([H])/C(C=O)=C\C[C@@]1([H])[C@](CC2)([C@@H](C)CC/C=C(C)\C)O)([C@](C)(O)CC3=O)[H]
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Structure Classification
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Initial Source
Cochliobolus heterostrophus.
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (124.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Gianani L, et al. Effects of ophiobolin B on cell enlargement and H(+)/K (+) exchange in maize coleoptile tissues. Planta. 1979 Jan;146(3):271-4. [Content Brief]
[2]. Krizsán K, et al. Effect of the sesterterpene-type metabolites, ophiobolins A and B, on zygomycetes fungi. FEMS Microbiol Lett. 2010 Dec;313(2):135-40. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4840 mL | 12.4202 mL | 24.8404 mL | 62.1010 mL |
| 5 mM | 0.4968 mL | 2.4840 mL | 4.9681 mL | 12.4202 mL | |
| 10 mM | 0.2484 mL | 1.2420 mL | 2.4840 mL | 6.2101 mL | |
| 15 mM | 0.1656 mL | 0.8280 mL | 1.6560 mL | 4.1401 mL | |
| 20 mM | 0.1242 mL | 0.6210 mL | 1.2420 mL | 3.1050 mL | |
| 25 mM | 0.0994 mL | 0.4968 mL | 0.9936 mL | 2.4840 mL | |
| 30 mM | 0.0828 mL | 0.4140 mL | 0.8280 mL | 2.0700 mL | |
| 40 mM | 0.0621 mL | 0.3105 mL | 0.6210 mL | 1.5525 mL | |
| 50 mM | 0.0497 mL | 0.2484 mL | 0.4968 mL | 1.2420 mL | |
| 60 mM | 0.0414 mL | 0.2070 mL | 0.4140 mL | 1.0350 mL | |
| 80 mM | 0.0311 mL | 0.1553 mL | 0.3105 mL | 0.7763 mL | |
| 100 mM | 0.0248 mL | 0.1242 mL | 0.2484 mL | 0.6210 mL |
Ophiobolin B Related Classifications
HY-N6780 Related Classifications
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