p-Nitrophenyl α-L-arabinopyranoside
p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection.
For research use only. We do not sell to patients.
- CAS No.: 1223-07-0
- Formula: C11H13NO7
- Molecular Weight:271.22
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
The affinity Km value of p-Nitrophenyl α-L-arabinopyranoside with rBgaA is 6.06 mM[1].
p-Nitrophenyl α-L-arabinopyranoside shows high activity to xylosidase–arabinosidase (xarB) gene[2].
Application of p-Nitrophenyl α-L-arabinopyranoside in the detection of enzyme activity[3]
(1) 200 μL 2 mM p-Nitrophenyl α- L-arabinopyranoside, 100 μL enzyme, 300 μL 50 mM phosphate buffer (pH 7.0) were incubated at 37°C for 0.5, 1, and 5 h.
(2) 400 μL 0.5 M NaOH was added to terminate the reaction.
(3) Measure the absorbance of the mixture at 405 nm.
Note: In the presence of ginsenosides, the reaction was terminated by extraction with butanol.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1223-07-0
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Molecular Weight 271.22
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Formula C11H13NO7
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SMILES
O[C@H]([C@H]([C@H](CO1)O)O)[C@@H]1OC2=CC=C([N+]([O-])=O)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Kosugi A, et al. Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002 Dec;184(24):6859-65. [Content Brief]
[2]. Mai V, et al. Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus. Gene. 2000 Apr 18;247(1-2):137-43. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
p-Nitrophenyl α-L-arabinopyranoside Related Classifications
HY-134429 Related Classifications
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