p38-α MAPK-IN-1
Based on 2 publication(s) in Google Scholar
p38-α MAPK-IN-1 is an inhibitor of MAPK14 (p38-α), with IC50 of 2300 nM in EFC displacement assay, and 5500 nM in HTRF assay.
For research use only. We do not sell to patients.
- Purity: 99.71%
- CAS No.: 443913-15-3
- Formula: C27H35N5O3
- Molecular Weight:477.60
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) p38-α MAPK-IN-1
More
Biological Activity
IC50: 2300 nM (p38-α, in EFC assay), 5500 nM (p38-α, in HTRF assay)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | EC50 |
6200 nM
Compound: 80
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Inhibition of LPS-induced tumor necrosis factor-alpha (TNF-alpha) production in THP-1 cells
Inhibition of LPS-induced tumor necrosis factor-alpha (TNF-alpha) production in THP-1 cells
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[PMID: 12086485] |
p38-α MAPK-IN-1 (Compound 12) is an inhibitor of MAPK14 (p38-α)>, with IC50 of 2300 nM in EFC displacement assay, and 5500 nM in HTRF assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 443913-15-3
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Appearance Solid
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Molecular Weight 477.60
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Formula C27H35N5O3
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Color White to off-white
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SMILES
CC1=CC=C(N2C(NC(NC3=CC=C(OCCN4CCOCC4)C=C3)=O)=CC(C(C)(C)C)=N2)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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ACS Nano
Small Gold Nanoparticles Alleviate Huntington's Disease via Modulating p38α Mitogen-Activated Protein Kinase and Pyruvate Dehydrogenase Kinase 1. [Abstract]2025 Dec 22. PMID: 41429604 -
Stem Cells
DAPK1 Interacts with the p38 Isoform MAPK14, Preventing Its Nuclear Translocation and Stimulation of Bone Marrow Adipogenesis. [Abstract]2022 May 27;40(5):508-522. PMID: 35403694
Solvent & Solubility
DMSO : 100 mg/mL (209.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0938 mL | 10.4690 mL | 20.9380 mL | 52.3451 mL |
| 5 mM | 0.4188 mL | 2.0938 mL | 4.1876 mL | 10.4690 mL | |
| 10 mM | 0.2094 mL | 1.0469 mL | 2.0938 mL | 5.2345 mL | |
| 15 mM | 0.1396 mL | 0.6979 mL | 1.3959 mL | 3.4897 mL | |
| 20 mM | 0.1047 mL | 0.5235 mL | 1.0469 mL | 2.6173 mL | |
| 25 mM | 0.0838 mL | 0.4188 mL | 0.8375 mL | 2.0938 mL | |
| 30 mM | 0.0698 mL | 0.3490 mL | 0.6979 mL | 1.7448 mL | |
| 40 mM | 0.0523 mL | 0.2617 mL | 0.5235 mL | 1.3086 mL | |
| 50 mM | 0.0419 mL | 0.2094 mL | 0.4188 mL | 1.0469 mL | |
| 60 mM | 0.0349 mL | 0.1745 mL | 0.3490 mL | 0.8724 mL | |
| 80 mM | 0.0262 mL | 0.1309 mL | 0.2617 mL | 0.6543 mL | |
| 100 mM | 0.0209 mL | 0.1047 mL | 0.2094 mL | 0.5235 mL |
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