pacFA 

pacFA is a bifunctional fatty acid with both photocrosslinking and click chemistry properties. pacFA can be metabolically incorporated into phospholipids by cells, enabling in-situ capture, identification and imaging of protein-lipid interactions in live cells and living organisms via ultraviolet crosslinking and click reactions^[1].

pacFA specifically binds to and labels the fatty acid carrier protein BSA via photoaffinity labeling, a process dependent on ultraviolet light and click chemistry, while it shows no labeling effect on lysozyme^[1].
pacFA (16 h) can be metabolically incorporated into all major classes of phospholipids in CHO and HeLa cells, but not into sphingolipids^[1].
pacFA (1-16 h) is rapidly metabolized and incorporated into neutral lipids and phospholipids in HeLa cells^[1].
pacFA (1 h) enables in-situ visualization of endoplasmic reticulum-localized protein-lipid interactions in UV-irradiated HeLa cells, while no labeling is detected under control conditions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

pacFA (feeding) enables visualization of protein-lipid interactions in C. elegans, with UV-dependent, tissue-specific fluorescent staining concentrated in intestinal and muscle cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

264.37

C₁₅H₂₄N₂O₂

1262788-55-5

O=C(O)CCCCCCCCC1(N=N1)CCCC#C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Haberkant P, et al. In vivo profiling and visualization of cellular protein–lipid interactions using bifunctional fatty acids. Angewandte Chemie International Edition. 2013 Apr 2;52(14):4033-8.