pacFA
pacFA is a bifunctional fatty acid with both photocrosslinking and click chemistry properties. pacFA can be metabolically incorporated into phospholipids by cells, enabling in-situ capture, identification and imaging of protein-lipid interactions in live cells and living organisms via ultraviolet crosslinking and click reactions.
For research use only. We do not sell to patients.
- CAS No.: 1262788-55-5
- Formula: C15H24N2O2
- Molecular Weight:264.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
pacFA specifically binds to and labels the fatty acid carrier protein BSA via photoaffinity labeling, a process dependent on ultraviolet light and click chemistry, while it shows no labeling effect on lysozyme[1].
pacFA (16 h) can be metabolically incorporated into all major classes of phospholipids in CHO and HeLa cells, but not into sphingolipids[1].
pacFA (1-16 h) is rapidly metabolized and incorporated into neutral lipids and phospholipids in HeLa cells[1].
pacFA (1 h) enables in-situ visualization of endoplasmic reticulum-localized protein-lipid interactions in UV-irradiated HeLa cells, while no labeling is detected under control conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1262788-55-5
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Molecular Weight 264.37
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Formula C15H24N2O2
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SMILES
O=C(O)CCCCCCCCC1(N=N1)CCCC#C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
pacFA Related Classifications
HY-W895360 Related Classifications
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