Parbendazole hydrochloride
Parbendazole hydrochloride (SKF‑29044 hydrochloride) is an orally active benzimidazole carbamate compound with multiple biological activities. Parbendazole hydrochloride binds to free tubulin and inhibits microtubule polymerization; it also activates the JNK/c‑Jun signaling axis and upregulates the expression of the downstream axonal repulsion factor Sema3A. Parbendazole hydrochloride upregulates KAL-1 mRNA expression, reduces nerve growth factor levels, and promotes the terminal differentiation of normal human epidermal keratinocytes. Parbendazole hydrochloride induces apoptosis in differentiated acute myeloid leukemia monocytes and exerts antileukemic activity in a mouse xenograft model of acute myeloid leukemia. Parbendazole hydrochloride exhibits broad-spectrum anthelmintic activity against various animal nematodes. Parbendazole hydrochloride is used in research related to acute myeloid leukemia and parasitic infections.
For research use only. We do not sell to patients.
- CAS No.: 83601-81-4
- Formula: C13H18ClN3O2
- Molecular Weight:283.75
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Biological Activity
Description
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JNK |
β-Tubulin |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| THP-1 | IC50 |
0.6 nM
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Inhibition of cell viability against THP-1 human acute myeloid leukemia cells assessed by WST assay after 48 h of incubation.
Inhibition of cell viability against THP-1 human acute myeloid leukemia cells assessed by WST assay after 48 h of incubation.
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38267545 |
| THP-1 | IC50 |
0.3 nM
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Inhibition of cell viability against THP-1 human acute myeloid leukemia cells assessed by WST assay after 72 h of incubation.
Inhibition of cell viability against THP-1 human acute myeloid leukemia cells assessed by WST assay after 72 h of incubation.
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38267545 |
In Vitro
Parbendazole (PBZ) hydrochloride exhibits extremely potent anti-survival activity against THP-1 acute myeloid leukemia cells, with an IC50 of 0.6 nM at 48 h and an IC50 of 0.3 nM at 72 h. At concentrations ≥50 nM, it effectively induces rapid monocytic differentiation of THP-1 acute myeloid leukemia cells, followed by apoptosis, which is accompanied by upregulation of the KLF4/DPYSL2A differentiation axis[1].
Parbendazole (100 nM; 48 h) hydrochloride exerts broad and potent differentiation-inducing and pro-apoptotic effects on acute myeloid leukemia cell lines with different subtypes and genetic backgrounds, while exhibiting limited toxicity to normal human hematopoietic progenitor cells; it effectively induces monocytic differentiation, triggers apoptosis, and upregulates the KLF4/DPYSL2A axis in primary human AML-PDX cells[1].
Parbendazole (0.01-1 μM; 48 h) hydrochloride induces the expression of the Sema3A gene and protein in normal human epidermal keratinocytes in a dose-dependent manner[2].
Parbendazole (1 μM; 48 h) hydrochloride downregulates NGF mRNA expression and upregulates KAL‑1 mRNA expression in normal human epidermal keratinocytes (NHEKs)[2].
Parbendazole (1 μM; 24 h) hydrochloride promotes the phosphorylation of JNK and c‑Jun in normal human epidermal keratinocytes (NHEKs)[2].
Parbendazole (1-10 μM; 24 h) hydrochloride increases the mRNA and protein expression levels of Sema3A in an in vitro reconstructed human epidermis (RHE) model[2].
Parbendazole hydrochloride: Wild-type Physarum polycephalum CLd-AXE tubulin completely inhibits in vitro microtubule assembly at 2-5 μM parbendazole, whereas mutant BEN210-AXE tubulin retains full microtubule assembly capacity even at parbendazole concentrations as high as 50 μM[3].
Parbendazole hydrochloride inhibits the growth of wild-type Physarum polycephalum CLd‑AXE myxamoebae and the in vitro assembly of their tubulin.
Parbendazole hydrochloride exhibits significant pH-dependent ultraviolet absorption characteristics, and shows a pronounced red shift in alkaline methanol medium compared to neutral and acidic methanol conditions[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Parbendazole (5 mg/kg; i.v.; single injection) hydrochloride exhibits rapid distribution, a long elimination half-life of 720.0 min, and very low total excretion via urine and feces over 5 days in healthy goats[4].
Parbendazole (100 mg/kg; p.o.; daily; 28 consecutive days) hydrochloride exerts significant in vivo anti-leukemic activity in the AML PDX model by reducing leukemia burden, inducing leukemic cell monocytic differentiation, and prolonging animal survival[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 83601-81-4
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Molecular Weight 283.75
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Formula C13H18ClN3O2
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SMILES
O=C(NC1=NC2=C(N1)C=CC(CCCC)=C2)OC.Cl
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Synonyms
SKF 29044 hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Matsuo H, et al. Parbendazole as a promising drug for inducing differentiation of acute myeloid leukemia cells with various subtypes. Communications biology. 2024 Jan 24;7(1):123. [Content Brief]
[3]. Foster KE, et al. A mutant beta-tubulin confers resistance to the action of benzimidazole-carbamate microtubule inhibitors both in vivo and in vitro. European journal of biochemistry. 1987 Mar 16;163(3):449-55. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Parbendazole hydrochloride Related Classifications
HY-115364A Related Classifications
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