Pinostrobin chalcone
Based on 1 Customer Validation
Pinostrobin chalcone is a chalcone found in Boesenbergia rotunda and Alpinia mutica. Pinostrobin chalcone exhibits cytotoxicity against various cancer cells. Pinostrobin chalcone is predicted to bind to the JAK active site, forming a hydrogen bond with Asn859 and hydrophobic interactions with aromatic residues. Pinostrobin chalcone can be used in research related to breast cancer, colon cancer, cervical cancer, and epidermoid carcinoma.
For research use only. We do not sell to patients.
- Purity : 99.94%
- CAS No.: 18956-15-5
- Formula: C16H14O4
- Molecular Weight:270.28
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-N0269
EchinatinAnti-Inflammatory Agent -
HY-N0231
BavachalconeBacterial Inhibitor -
HY-N3057R
Pinostrobin chalcone (Standard)Reference Standard -
HY-N0372
Licochalcone AUGTs inhibitor -
HY-N4187
Licochalcone DNF-κB p65 Inhibitor -
HY-N0231R
Bavachalcone (Standard)Bacterial Inhibitor -
HY-N0374
Licochalcone Cα-Glucosidase Inhibitor -
HY-128400
4'-MethoxychalconePARP Activator -
HY-N8786
2,4,4'-TrihydroxydihydrochalconeNatural Product -
HY-N0372R
Licochalcone A (Standard)UGTs inhibitor -
HY-N0269R
Echinatin (Standard)Anti-Inflammatory Agent -
HY-128400R
4'-Methoxychalcone (Standard)PARP Activator
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| MDA-MB-231 | IC50 |
55.41 μM
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Cytotoxic activity against human MDA-MB-231 triple-negative breast cancer cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxic activity against human MDA-MB-231 triple-negative breast cancer cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
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2210315509666190117151542 |
| HT-29 | IC50 |
83.54 μM
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Cytotoxic activity against human HT-29 colon cancer cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
Cytotoxic activity against human HT-29 colon cancer cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.
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2210315509666190117151542 |
| KB | IC50 |
6.2 μg/mL
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Cytotoxicity against human KB epidermoid carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
Cytotoxicity against human KB epidermoid carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
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21240148 |
| MCF7 | IC50 |
7.3 μg/mL
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Cytotoxicity against human MCF7 hormone-dependent breast carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
Cytotoxicity against human MCF7 hormone-dependent breast carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
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21240148 |
| Ca-Ski | IC50 |
7.7 μg/mL
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Cytotoxicity against human CaSki cervical carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
Cytotoxicity against human CaSki cervical carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
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21240148 |
| MRC5 | IC50 |
7.9 μg/mL
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Cytotoxicity against non-human MRC5 fibroblast cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
Cytotoxicity against non-human MRC5 fibroblast cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
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21240148 |
| HCT-116 | IC50 |
54.1 μg/mL
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Cytotoxicity against human HCT116 colon carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
Cytotoxicity against human HCT116 colon carcinoma cells assessed as inhibition rate incubated for 48 to 72 hrs by neutral red assay.
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21240148 |
In Vitro
Pinostrobin chalcone (48 h) exhibits cytotoxic activity against MDA-MB-231 and HT-29 cancer cell lines, with IC50 values of 55.41 µM and 83.54 µM, respectively[1].
Pinostrobin chalcone (48-72 h) exhibits significant cytotoxic activity against KB, MCF7, and CaSki human cancer cell lines with IC50 values of 6.2, 7.3, and 7.7 µg/mL, respectively, and also shows cytotoxicity against normal MRC5 cells with an IC50 of 7.9 µg/mL[2].
Pinostrobin chalcone docking predicts binding to human Janus kinase (JAK2) via hydrogen bonds mediated by its methoxy and hydroxyl groups, with the 3-position OH group forming a hydrogen bond with Asn859[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 18956-15-5
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Appearance Solid
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Molecular Weight 270.28
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Formula C16H14O4
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Color Orange to red
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SMILES
O=C(C1=C(C=C(OC)C=C1O)O)/C=C/C2=CC=CC=C2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (369.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6999 mL | 18.4993 mL | 36.9987 mL | 92.4967 mL |
| 5 mM | 0.7400 mL | 3.6999 mL | 7.3997 mL | 18.4993 mL | |
| 10 mM | 0.3700 mL | 1.8499 mL | 3.6999 mL | 9.2497 mL | |
| 15 mM | 0.2467 mL | 1.2333 mL | 2.4666 mL | 6.1664 mL | |
| 20 mM | 0.1850 mL | 0.9250 mL | 1.8499 mL | 4.6248 mL | |
| 25 mM | 0.1480 mL | 0.7400 mL | 1.4799 mL | 3.6999 mL | |
| 30 mM | 0.1233 mL | 0.6166 mL | 1.2333 mL | 3.0832 mL | |
| 40 mM | 0.0925 mL | 0.4625 mL | 0.9250 mL | 2.3124 mL | |
| 50 mM | 0.0740 mL | 0.3700 mL | 0.7400 mL | 1.8499 mL | |
| 60 mM | 0.0617 mL | 0.3083 mL | 0.6166 mL | 1.5416 mL | |
| 80 mM | 0.0462 mL | 0.2312 mL | 0.4625 mL | 1.1562 mL | |
| 100 mM | 0.0370 mL | 0.1850 mL | 0.3700 mL | 0.9250 mL |
Pinostrobin chalcone Related Classifications
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