PKCδ Peptide Substrate acetate
PKCδ Peptide Substrate acetate is a highly selective peptide substrate targeting PKCδ. PKCδ Peptide Substrate acetate can only be efficiently catalyzed for phosphorylation modification by PKCδ, and Thr431 is the sole phosphorylation site. PKCδ Peptide Substrate acetate binds to the catalytic pocket of PKC in a competitive manner, but exhibits no PKC agonistic or inhibitory activity. PKCδ Peptide Substrate acetate is a dedicated biochemical probe for the quantitative detection of PKCδ in vitro, and can be used for studies on PKCδ-related pathways.
For research use only. We do not sell to patients.
- Formula: C109H191N35O29S·xC2H4O2
- Molecular Weight:2487.96 (free base)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PKCδ Peptide Substrate acetate acts as a selective, high-affinity substrate for recombinant human PKCδ (expressed in Sf9 cells) with a Km of 0.98 μM, and is a poorer substrate for other recombinant human PKC isozymes including PKCα, PKCβI, and PKCζ[2].
The PKCδ Peptide Substrate acetate is an absolutely specific substrate for PKCδ, exhibiting efficient phosphorylation by PKCδ with a Km of 22.9 μM, while showing no significant phosphorylation by cPKC, PKCβ, PKCγ, PKCε, PKCη, or PKCζ, and only minimal phosphorylation by PKCα[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 2487.96 (free base)
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Formula C109H191N35O29S·xC2H4O2
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SMILES
O=C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](C)C(N[C@@H](C(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(O)=O)C(N[C@@H](CCSC)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCC(N)=O)C(N[C@@H]([C@H](O)C)C(N[C@@H](C(C)C)C(N[C@@H](C)C(N[C@@H](C(C)C)C(NCC(N[C@@H](C(C)C)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CCCCN)C(N[C@@H](C)C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CCCNC(N)=N)N.CC(O)=O.[x]
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Sequence
Arg-Phe-Ala-Val-Arg-Asp-Met-Arg-Gln-Thr-Val-Ala-Val-Gly-Val-Ile-Lys-Ala-Val-Asp-Lys-Lys
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Sequence Shortening
RFAVRDMRQTVAVGVIKAVDKK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Kang JH. Protein Kinase C (PKC) Isozymes and Cancer. New Journal of Science. 2014;2014(1):231418.
[2]. Nishikawa K, Toker A, Johannes FJ, et al.. Determination of the specific substrate sequence motifs of protein kinase C isozymes. The Journal of biological chemistry. 1997 Jan 10;272(2):952-60. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PKCδ Peptide Substrate acetate Related Classifications
HY-P3818A Related Classifications
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