PKCδ Peptide Substrate
PKCδ Peptide Substrate is a highly selective peptide substrate targeting PKCδ. PKCδ Peptide Substrate can only be efficiently catalyzed for phosphorylation modification by PKCδ, and Thr431 is the sole phosphorylation site. PKCδ Peptide Substrate binds to the catalytic pocket of PKC in a competitive manner, but exhibits no PKC agonistic or inhibitory activity. PKCδ Peptide Substrate is a dedicated biochemical probe for the quantitative detection of PKCδ in vitro, and can be used for studies on PKCδ-related pathways.
For research use only. We do not sell to patients.
- CAS No.: 813416-46-5
- Formula: C109H191N35O29S
- Molecular Weight:2487.96
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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PKCδ 0.98 μM (Km) |
PKCδ 22.9 (Km) |
PKCδ Peptide Substrate acts as a selective, high-affinity substrate for recombinant human PKCδ (expressed in Sf9 cells) with a Km of 0.98 μM, and is a poorer substrate for other recombinant human PKC isozymes including PKCα, PKCβI, and PKCζ[2].
The PKCδ Peptide Substrate is an absolutely specific substrate for PKCδ, exhibiting efficient phosphorylation by PKCδ with a Km of 22.9 μM, while showing no significant phosphorylation by cPKC, PKCβ, PKCγ, PKCε, PKCη, or PKCζ, and only minimal phosphorylation by PKCα[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 813416-46-5
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Molecular Weight 2487.96
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Formula C109H191N35O29S
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Sequence
Arg-Phe-Ala-Val-Arg-Asp-Met-Arg-Gln-Thr-Val-Ala-Val-Gly-Val-Ile-Lys-Ala-Val-Asp-Lys-Lys
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Sequence Shortening
RFAVRDMRQTVAVGVIKAVDKK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
|---|---|---|
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
|---|---|
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
References
[1]. Kang JH. Protein Kinase C (PKC) Isozymes and Cancer. New Journal of Science. 2014;2014(1):231418.
[2]. Nishikawa K, Toker A, Johannes FJ, et al.. Determination of the specific substrate sequence motifs of protein kinase C isozymes. The Journal of biological chemistry. 1997 Jan 10;272(2):952-60. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PKCδ Peptide Substrate Related Classifications
HY-P3818 Related Classifications
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