Poly(ethylene glycol) dithiol (Mn 3400)

Poly (ethylene glycol) dithiol (Mn 3400) (PEG2SH 3400; compound 4) participates as a dithiol monomer in the step-growth polymerization of hexahydrotriazine (HT) to generate PEG-based poly (thioaminal) 4a^[1].
When poly (ethylene glycol) dithiol (Mn 3400) (7.5% w/v thiol-ene formulation; 2 mol% LAP; thiol:ene = 1:1; UV-A 365 nm, 25 mW/cm², 10 min; 1-21 d) is used to construct gel-NB/PEG2SH 3400 hydrogels for encapsulation of GFP-labeled hASC/TERT1 cells, the cells undergo matrix remodeling with prolonged culture time; compared with low molecular weight crosslinking systems such as DTT and TEG2SH, remodeling occurs later in high molecular weight crosslinking systems^[2].
When gel-NB/PEG2SH 3400 hydrogels constructed with Poly (ethylene glycol) dithiol (Mn 3400) (7.5% w/v thiol-ene formulation; 2 mol% LAP; thiol:ene = 1:1; UV-A 365 nm, 25 mW/cm², 10 min; 1-14 d) encapsulate GFP-labeled hASC/TERT1 cells, the metabolic activity on day 1 is comparable to that of gel-MOD; on days 2 and 4, the metabolic activity of PEG-containing formulations is lower than that of gel-MOD, while no significant differences are observed among different thiol-ene systems; on day 8, no significant differences exist between thiol-ene systems and gel-MOD^[2].
Poly (ethylene glycol) dithiol (Mn 3400) (PEG2SH 3400; corresponding to component concentrations of 10, 7.5, and 5% w/v gelatin formulations; 2 h; continued incubation for 24 h after removal of the component) does not exhibit the dark toxicity observed with low-molecular-weight DTT and TEG2SH in GFP-labeled hASC/TERT1 cells^[2].

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