Preparation of highly purified momordin II without ribonuclease activity
- Life Sci. 1999;65(14):1485-91. doi: 10.1016/s0024-3205(99)00389-6.
Affiliations
- 1. Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
PMID: 10530800
DOI: 10.1016/s0024-3205(99)00389-6
Abstract
Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribosomes and from DNA, and had no RNase activity.
Products
-
Cat. No.Product NameDescriptionTargetResearch Area
-
target: DNA/RNA SynthesisResearch Areas: Metabolic Disease