Hypertonic sabouraud broth as a simple and powerful test for Candida dubliniensis screening

  • Diagn Microbiol Infect Dis. 2002 May;43(1):85-6. doi: 10.1016/s0732-8893(02)00368-1.
Sydney Hartz Alves  1 ,  Eveline Pipolo Milan ,  Priscilla de Laet Sant'Ana ,  Loiva O Oliveira ,  Janio M Santurio ,  Arnaldo Lopes Colombo
Affiliations
  • 1. Department of CIinical and Toxicological Analysis, Universidade Federal de Santa Maria, RS, Santa Maria, Brazil.
PMID: 12052633 DOI: 10.1016/s0732-8893(02)00368-1
Abstract

We developed a new screening test for C. dubliniensis based on its inability to grow on Sabouraud dextrose broth with 6.5% NaCl. A total of 266 clinical yeast isolates and 3 reference strains were tested, including 250 C. albicans and 19 C. dubliniensis strains. All C. albicans isolates tested exhibited significant growth on hypertonic Sabouraud broth up to 96 h, while, all C. dubliniensis isolates did not exhibit any visually detectable growth during the same period.

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