Characterization of enzymatic D-xylulose 5-phosphate synthesis

  • Biotechnol Bioeng. 2008 Nov 1;101(4):761-7. doi: 10.1002/bit.21949.
Jobin Shaeri  1 ,  Ian Wright ,  Elner B Rathbone ,  Roland Wohlgemuth ,  John M Woodley
Affiliations
  • 1. Department of Biochemical Engineering, University College London, Torrington Place, London, UK.
PMID: 18553501 DOI: 10.1002/bit.21949
Abstract

In this article we report on the characterization of the enzymatic synthesis of D-xylulose 5-phosphate using triosephosphate isomerase and Transketolase. Two potential starting substrates are possible with this scheme. The data presented here allow a comparison of both routes for the synthesis, based on experimental information on reaction kinetics. Operational guidelines are proposed which should assist in the scale-up of such syntheses.

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