Probing key targets in insulin signaling and adipogenesis using a methanolic extract of Costus pictus and its bioactive molecule, methyl tetracosanoate

  • Biotechnol Lett. 2009 Dec;31(12):1837-41. doi: 10.1007/s10529-009-0105-3.
Kusampudi Shilpa  1 ,  Kadapakkam Nandabalan Sangeetha ,  Velusamy Shanmuganathan Muthusamy ,  Sundaresan Sujatha ,  Baddireddi Subadhra Lakshmi
Affiliations
  • 1. Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, 600025, India.
PMID: 19693444 DOI: 10.1007/s10529-009-0105-3
Abstract

A methanolic extract of Costus pictus (CPME) showed optimum anti-diabetic activity at 100 ng/ml. Bioactivity-guided purification of CPME led to the isolation of methyl tetracosanoate (MT) which showed an optimum glucose uptake at 1 ng/ml. CPME at 10 mug/ml inhibited adipogenesis whereas fully differentiated adipocytes exhibited a 3-fold increase in lipid accumulation compared to pre-adipocytes. Gene and protein expression of key targets in Insulin signaling and adipogenesis pathway revealed that CPME exhibited anti-diabetic activity along with anti-adipogenic activity whereas MT demonstrated only anti-diabetic activity.

Products