A chemical platform for improved induction of human iPSCs

  • Nat Methods. 2009 Nov;6(11):805-8. doi: 10.1038/nmeth.1393.
Tongxiang Lin  1 ,  Rajesh Ambasudhan ,  Xu Yuan ,  Wenlin Li ,  Simon Hilcove ,  Ramzey Abujarour ,  Xiangyi Lin ,  Heung Sik Hahm ,  Ergeng Hao ,  Alberto Hayek ,  Sheng Ding
Affiliations
  • 1. Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA.
PMID: 19838168 DOI: 10.1038/nmeth.1393
Abstract

The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

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