Oligosaccharide-assisted direct immunosensing of small molecules

  • Anal Chem. 2010 Jun 1;82(11):4333-6. doi: 10.1021/ac100865p.
Norihiro Kobayashi  1 ,  Hiroyuki Oyama ,  Iwao Suzuki ,  Yoshinori Kato ,  Takeo Umemura ,  Junichi Goto
Affiliations
  • 1. Kobe Pharmaceutical University, 4-19-1, Motoyama-Kitamachi, Higashinada-ku, Kobe 658-8558, Japan. [email protected]
PMID: 20459059 DOI: 10.1021/ac100865p
Abstract

"Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.

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