Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells

  • FEBS Lett. 2010 Nov 5;584(21):4389-95. doi: 10.1016/j.febslet.2010.09.047.
Sei-ichi Sato  1 ,  Hiroki Inoue ,  Takeshi Kogure ,  Mitsuo Tagaya ,  Katsuko Tani
Affiliations
  • 1. School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.
PMID: 20932832 DOI: 10.1016/j.febslet.2010.09.047
Abstract

Mammals have three members of the intracellular Phospholipase A(1) protein family (phosphatidic acid preferring-phospholipase A(1), p125, and KIAA0725p). In this study, we showed that KIAA0725p is localized in the Golgi, and is rapidly cycled between the Golgi and cytosol. Catalytic activity is important for targeting of KIAA0725p to Golgi membranes. RNA interference experiments suggested that KIAA0725p contributes to efficient membrane trafficking from the Golgi apparatus to the plasma membrane, but is not involved in brefeldin A-induced Golgi-to-endoplasmic reticulum retrograde transport.